@article{PAR00015081,
  title = {{A} robust method for the rapid generation of recombinant {Z}ika virus expressing the {GFP} reporter gene},
  author = {{G}adea, {G}. and {B}os, {S}. and {K}rejbich-{T}rotot, {P}. and {C}lain, {E}. and {V}iranaicken, {W}. and {E}l-{K}alamouni, {C}. and {M}avingui, {P}atrick and {D}espres, {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{Z}ika virus ({ZIKV}) infection is a major public health problem with severe human congenital and neurological anomalies. {T}he screening of anti-{ZIKV} compounds and neutralizing antibodies needs reliable and rapid virus-based assays. {H}ere, we described a convenient method leading to the rapid production of molecular clones of {ZIKV}. {T}o generate a molecular clone of {ZIKV} strain {MR}766({NIID}), the viral genome was directly assembled into {V}ero cells after introduction of four overlapping synthetic fragments that cover the full-length genomic {RNA} sequence. {S}uch strategy has allowed the production of a recombinant {ZIKV} expressing the {GFP} reporter gene that is stable over two culturing rounds on {V}ero cells. {O}ur data demonstrate that the {ZIKV} reporter virus is a very reliable {GFP}-based tool for analyzing viral growth and measuring the neutralizing antibody as well as rapid screening of antiviral effect of different classes of inhibitors.},
  keywords = {{A}rbovirus ; {E}merging disease ; {F}lavivirus ; {Z}ika virus ; {M}olecular clones ; {R}ecombinant virus ; {GFP} reporter},
  booktitle = {},
  journal = {{V}irology},
  volume = {497},
  numero = {},
  pages = {157--162},
  ISSN = {0042-6822},
  year = {2016},
  DOI = {10.1016/j.virol.2016.07.015},
  URL = {https://www.documentation.ird.fr/hor/{PAR}00015081},
}