%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Gadea, G.
%A Bos, S.
%A Krejbich-Trotot, P.
%A Clain, E.
%A Viranaicken, W.
%A El-Kalamouni, C.
%A Mavingui, Patrick
%A Despres, P.
%T A robust method for the rapid generation of recombinant Zika virus expressing the GFP reporter gene
%D 2016
%L PAR00015081
%G ENG
%J Virology
%@ 0042-6822
%K Arbovirus ; Emerging disease ; Flavivirus ; Zika virus ; Molecular clones ; Recombinant virus ; GFP reporter
%M ISI:000383922200016
%P 157-162
%R 10.1016/j.virol.2016.07.015
%U https://www.documentation.ird.fr/hor/PAR00015081
%V 497
%W Horizon (IRD)
%X Zika virus (ZIKV) infection is a major public health problem with severe human congenital and neurological anomalies. The screening of anti-ZIKV compounds and neutralizing antibodies needs reliable and rapid virus-based assays. Here, we described a convenient method leading to the rapid production of molecular clones of ZIKV. To generate a molecular clone of ZIKV strain MR766(NIID), the viral genome was directly assembled into Vero cells after introduction of four overlapping synthetic fragments that cover the full-length genomic RNA sequence. Such strategy has allowed the production of a recombinant ZIKV expressing the GFP reporter gene that is stable over two culturing rounds on Vero cells. Our data demonstrate that the ZIKV reporter virus is a very reliable GFP-based tool for analyzing viral growth and measuring the neutralizing antibody as well as rapid screening of antiviral effect of different classes of inhibitors.
%$ 052 ; 020