Caputo A., Merhej V., Georgiades K., Fournier P. E., Croce O., Robert C., Raoult Didier. (2015). Pan-genomic analysis to redefine species and subspecies based on quantum discontinuous variation : the Klebsiella paradigm. Biology Direct, 10, p. art. 55. ISSN 1745-6150.
Titre du document
Pan-genomic analysis to redefine species and subspecies based on quantum discontinuous variation : the Klebsiella paradigm
Caputo A., Merhej V., Georgiades K., Fournier P. E., Croce O., Robert C., Raoult Didier
Source
Biology Direct, 2015,
10, p. art. 55 ISSN 1745-6150
Background: Various methods are currently used to define species and are based on the phylogenetic marker 16S ribosomal RNA gene sequence, DNA-DNA hybridization and DNA GC content. However, these are restricted genetic tools and showed significant limitations. Results: In this work, we describe an alternative method to build taxonomy by analyzing the pan-genome composition of different species of the Klebsiella genus. Klebsiella species are Gram-negative bacilli belonging to the large Enterobacteriaceae family. Interestingly, when comparing the core/pan-genome ratio; we found a clear discontinuous variation that can define a new species. Conclusions: Using this pan-genomic approach, we showed that Klebsiella pneumoniae subsp. ozaenae and Klebsiella pneumoniae subsp. rhinoscleromatis are species of the Klebsiella genus, rather than subspecies of Klebsiella pneumoniae. This pan-genomic analysis, helped to develop a new tool for defining species introducing a quantic perspective for taxonomy.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Biotechnologies [084]
