%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Caputo, A.
%A Merhej, V.
%A Georgiades, K.
%A Fournier, P. E.
%A Croce, O.
%A Robert, C.
%A Raoult, Didier
%T Pan-genomic analysis to redefine species and subspecies based on quantum discontinuous variation : the Klebsiella paradigm
%D 2015
%L PAR00013734
%G ENG
%J Biology Direct
%@ 1745-6150
%K Pan-genome ; Klebsiella pneumoniae ; Taxonomy
%M ISI:000361938300001
%P art. 55
%R 10.1186/s13062-015-0085-2
%U https://www.documentation.ird.fr/hor/PAR00013734
%V 10
%W Horizon (IRD)
%X Background: Various methods are currently used to define species and are based on the phylogenetic marker 16S ribosomal RNA gene sequence, DNA-DNA hybridization and DNA GC content. However, these are restricted genetic tools and showed significant limitations. Results: In this work, we describe an alternative method to build taxonomy by analyzing the pan-genome composition of different species of the Klebsiella genus. Klebsiella species are Gram-negative bacilli belonging to the large Enterobacteriaceae family. Interestingly, when comparing the core/pan-genome ratio; we found a clear discontinuous variation that can define a new species. Conclusions: Using this pan-genomic approach, we showed that Klebsiella pneumoniae subsp. ozaenae and Klebsiella pneumoniae subsp. rhinoscleromatis are species of the Klebsiella genus, rather than subspecies of Klebsiella pneumoniae. This pan-genomic analysis, helped to develop a new tool for defining species introducing a quantic perspective for taxonomy.
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