Charbonnel E., Benoit L., Nidelet S., Ortega Abboud Enrique, Gschloessl B., Leblois R., Ouvrard D., Chapuis M.P. (2025). Optimization and evaluation of the bestRAD sequencing approach : towards ascertainment of the invasion routes of the Oriental fruit fly, Bactrocera dorsalis. Molecular Ecology Resources, 25 (8), e14114 [15 p.]. ISSN 1755-098X.
Titre du document
Optimization and evaluation of the bestRAD sequencing approach : towards ascertainment of the invasion routes of the Oriental fruit fly, Bactrocera dorsalis
Année de publication
2025
Auteurs
Charbonnel E., Benoit L., Nidelet S., Ortega Abboud Enrique, Gschloessl B., Leblois R., Ouvrard D., Chapuis M.P.
Source
Molecular Ecology Resources, 2025,
25 (8), e14114 [15 p.] ISSN 1755-098X
The bestRAD technique is a reduced genome representation approach with high-capacity sample multiplexing and physical isolation of biotin-labelled target DNA fragments using streptavidin beads, which should reduce total cost and genotyping errors. While we here formalise the relevance of this approach within the HTS landscape, our foremost aim was to improve its replicability, validity, and transparency. We first optimised the molecular laboratory protocol and shared the associated protocols (e.g., final detailed methodologies, quality control, best practices) under the FAIR principles. Using 84 worldwide individual samples of the Oriental fruit fly, Bactrocera dorsalis, a major invasive pest, we revealed a low rate of PCR duplicates, robustness to DNA quality and quantity, high genotype call rate, insignificant genotyping error rate, high nuclear and mitochondrial genome representativeness, and a high level of genetic information. This in-depth data quality assessment, along with total cost and handling time reduced by an estimated one-third relative to the parent RAD-Seq version, demonstrates that bestRAD is an excellent compromise between cost and quality. While we generated high-quality genomic resources for B. dorsalis, we also share details and recommendations for the bestRAD technique that can be readily used in any laboratory and applied to all organisms, even without published genome sequence.
Plan de classement
Biochimie [020BIOCHI]
;
Environnement, écologie générale [021ENVECO]
;
Ecologie du parasitisme (sol, climat ~) [076MALPLA04]
;
Ravageurs des plantes [076RAVPLA]
;
Invertébrés [082FAUNE01]
Localisation
Fonds IRD [F B010095218]
Identifiant IRD
fdi:010095218
