Medkour H., Pruvost Lauryne, Miot E. F., Gong X. Q., Vaissayre Virginie, Tavadia Mihra, Boutinaud Pascal, Revel J., Hitakarun A., Sornjai W., Smith D. R., Nisole S., Hoen E. N. T., Bertrand-Michel J., Missé Dorothée, Marti G., Pompon Julien. (2025). Sphingomyelins in mosquito saliva reconfigure skin lipidome to promote viral protein levels and enhance transmission of flaviviruses. Cell Metabolism, 37 (7), p. [26 p.]. ISSN 1550-4131.
Titre du document
Sphingomyelins in mosquito saliva reconfigure skin lipidome to promote viral protein levels and enhance transmission of flaviviruses
Année de publication
2025
Auteurs
Medkour H., Pruvost Lauryne, Miot E. F., Gong X. Q., Vaissayre Virginie, Tavadia Mihra, Boutinaud Pascal, Revel J., Hitakarun A., Sornjai W., Smith D. R., Nisole S., Hoen E. N. T., Bertrand-Michel J., Missé Dorothée, Marti G., Pompon Julien
Source
Cell Metabolism, 2025,
37 (7), p. [26 p.] ISSN 1550-4131
Many flaviviruses with high pandemic potential are transmitted through mosquito bites. While mosquito saliva is essential for transmission and represents a promising pan-flaviviral target, there is a dearth of knowledge on salivary metabolic transmission enhancers. Here, we show that extracellular vesicle (EV)-derived sphingomyelins in mosquito saliva reconfigure the human cell lipidome to increase viral protein levels, boosting skin infection and enhancing transmission for flaviviruses. Lipids within internalized mosquito EVs enhance infection in fibroblast and immune human primary cells for multiple flaviviruses. Mosquito EV lipids selectively increase viral translation by inhibiting infection-induced endoplasmic reticulum (ER)-associated degradation of viral proteins. Infection enhancement solely results from the sphingomyelins within salivary mosquito EVs that augment human cell sphingomyelin concentration. Finally, EV-lipid co-inoculation exacerbates disease severity in vivo in mouse transmission assays. By discovering and elucidating how metabolic components of mosquito saliva promote transmission of flaviviruses, our study unveils lipids as a new category of targets against vectored transmission.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Entomologie médicale / Parasitologie / Virologie [052]
Localisation
Fonds IRD [F B010094385]
Identifiant IRD
fdi:010094385
