Dambrun M., Sare N., Vianou B., Amagbegnon R., Fievet Nadine, Massougbodji A., Houze S., Migot Nabias Florence. (2023). Serological diagnosis of toxoplasmosis in pregnancy : comparison between a manual commercial ELISA assay and the automated VIDAS (R) kit. European Journal of Clinical Microbiology and Infectious Diseases, 42, p. 759-769. ISSN 0934-9723.
Titre du document
Serological diagnosis of toxoplasmosis in pregnancy : comparison between a manual commercial ELISA assay and the automated VIDAS (R) kit
Dambrun M., Sare N., Vianou B., Amagbegnon R., Fievet Nadine, Massougbodji A., Houze S., Migot Nabias Florence
Source
European Journal of Clinical Microbiology and Infectious Diseases, 2023,
42, p. 759-769 ISSN 0934-9723
Knowledge of the toxoplasmosis serological status in pregnant women is important to allow adequate management for the prevention of congenital toxoplasmosis of those who are not immunized. Serological screening is generally carried out using commercial kits to determine the presence or absence of immunoglobulins M or G in the maternal blood. Robust results are therefore needed. We evaluated the performances of a commercial ELISA assay composed of several recombinant parasite antigens and of a commercial assay using parasite lysate to determine the serological status against Toxoplasma gondii of African pregnant women. A recruitment of 106 pregnant women during their third trimester of pregnancy was carried out in Benin. Serologies were performed with recomWell Toxoplasma IgM and IgG kits. Subsequently, the serological assays were carried out by an automaton method with the VIDAS (R) TOXO IgM and IgG II kits. Here we compared recomWell Toxoplasma to -VIDAS (R) TOXO results. Reproducibility tests of the recomWell kits were assessed following the discrepancies observed in the results. Of 106 plasmas tested, 47 showed anti-T. gondii IgG ( 44.3%), including 5 with IgM and high IgG avidity (4.7%). Of the two techniques, VIDAS (R) TOXO was more robust and specific for IgG while the recomWell Toxoplasma gave more false positive results. The combination of several techniques for the determination of serological toxoplasmosis status remains relevant. Methods using native proteins are closer to the reality of the environment. Therefore, kits using recombinant proteins should be tested on highly geographically diverse populations to refine their composition.
