Innovative integrated system for real-time measurement of hybridization and melting on standard format microarrays

2008

Article référencé dans le Web of Science WOS:000263637800009

Marcy Y., Cousin P. Y., Rattier M., Cerovic G., Escalier G., Béna Gilles, Gueron M., McDonagh L., le Boulaire F., Benisty H., Weisbuch C., Avarre Jean-Christophe

Biotechniques, 2008, 44 (7), p. 913-920 ISSN 0736-6205

Despite the great popularity and potential of microarrays, their use for research and clinical applications is still hampered by lengthy and costly design and optimization processes, mainly because the technology relies oil the end point measurement of hybridization. Thus, the ability to monitor many hybridization events oil a standard microarray slide in real time would greatly expand the use and benefit of this technology, as it would give access to better prediction of probe performance and improved optimization of hybridization parameters. Although real-time hybridization and thermal denaturation measurements have been reported, a complete walk-away system compatible with the standard format of microarrays is still unavailable. To address this issue, we have designed a biochip tool that combines a hybridization station with active mixing capability and temperature control together with a fluorescence reader in a single compact benchtop instrument. This integrated live It hybridization machine (LHM) allows measuring in real time the hybridization of target DNA to thousands of probes simultaneously and provides excellent levels of detection and superior sequence discrimination. Here we show on all environmental single nucleotide polymorphism (SNP) model system that the LHM enables a variety of experiments unachievable with conventional biochip tools.

Sciences fondamentales / Techniques d'analyse et de recherche [020]

Fonds IRD [F B010083464]

fdi:010083464