Ngom M., Cissoko M., Gray K., Hocher Valérie, Laplaze Laurent, Sy M.O., Svistoonoff Sergio, Champion Antony. (2020). Establishment of actinorhizal symbiosis in response to ethylene, salicylic acid, and jasmonate. In :
Champion Antony (ed.), Laplaze Laurent (ed.). Jasmonate in plant biology : methods and protocols. New York : Humana Press, 117-130. (Methods in Molecular Biology). ISBN 978-1-0716-0141-9.
Titre du document
Establishment of actinorhizal symbiosis in response to ethylene, salicylic acid, and jasmonate
Année de publication
2020
Auteurs
Ngom M., Cissoko M., Gray K., Hocher Valérie, Laplaze Laurent, Sy M.O., Svistoonoff Sergio, Champion Antony
In
Champion Antony (ed.), Laplaze Laurent (ed.), Jasmonate in plant biology : methods and protocols
Source
New York : Humana Press, 2020,
117-130 (Methods in Molecular Biology). ISBN 978-1-0716-0141-9
Phytohormones play a crucial role in regulating plant developmental processes. Among them, ethylene and jasmonate are known to be involved in plant defense responses to a wide range of biotic stresses as their levels increase with pathogen infection. In addition, these two phytohormones have been shown to inhibit plant nodulation in legumes. Here, exogenous salicylic acid (SA), jasmonate acid (JA), and ethephon (ET) were applied to the root system of Casuarina glauca plants before Frankia inoculation, in order to analyze their effects on the establishment of actinorhizal symbiosis. This protocol further describes how to identify putative ortholog genes involved in ethylene and jasmonate biosynthesis and/or signaling pathways in plant, using the Arabidopsis Information Resource (TAIR), Legume Information System (LIS), and Genevestigator databases. The expression of these genes in response to the bacterium Frankia was analyzed using the gene atlas for Casuarina-Frankia symbiosis (SESAM web site).
Plan de classement
Sciences du monde végétal [076]
;
Biotechnologies [084]
Localisation
Fonds IRD [F A010082337]
Identifiant IRD
fdi:010082338
