Kiwuka C., Goudsmit E., Tournebize R., de Aquino S. O., Douma J. C., Bellanger L., Crouzillat D., Stoffelen P., Sumirat U., Legnate H., Marraccini P., Kochko Alexandre de, Andrade A. C., Mulumba J. W., Musoli P., Anten N. P. R., Poncet Valérie. (2021). Genetic diversity of native and cultivated Ugandan Robusta coffee (Coffea canephora Pierre ex A. Froehner) : climate influences, breeding potential and diversity conservation. PLoS One, 16 (2), p. e0245965 [19 p.]. ISSN 1932-6203.
Titre du document
Genetic diversity of native and cultivated Ugandan Robusta coffee (Coffea canephora Pierre ex A. Froehner) : climate influences, breeding potential and diversity conservation
Année de publication
2021
Auteurs
Kiwuka C., Goudsmit E., Tournebize R., de Aquino S. O., Douma J. C., Bellanger L., Crouzillat D., Stoffelen P., Sumirat U., Legnate H., Marraccini P., Kochko Alexandre de, Andrade A. C., Mulumba J. W., Musoli P., Anten N. P. R., Poncet Valérie
Source
PLoS One, 2021,
16 (2), p. e0245965 [19 p.] ISSN 1932-6203
Wild genetic resources and their ability to adapt to environmental change are critically important in light of the projected climate change, while constituting the foundation of agricultural sustainability. To address the expected negative effects of climate change on Robusta coffee trees (Coffea canephora), collecting missions were conducted to explore its current native distribution in Uganda over a broad climatic range. Wild material from seven forests could thus be collected. We used 19 microsatellite (SSR) markers to assess genetic diversity and structure of this material as well as material from two ex-situ collections and a feral population. The Ugandan C. canephora diversity was then positioned relative to the species' global diversity structure. Twenty-two climatic variables were used to explore variations in climatic zones across the sampled forests. Overall, Uganda's native C. canephora diversity differs from other known genetic groups of this species. In northwestern (NW) Uganda, four distinct genetic clusters were distinguished being from Zoka, Budongo, Itwara and Kibale forests A large southern-central (SC) cluster included Malabigambo, Mabira, and Kalangala forest accessions, as well as feral and cultivated accessions, suggesting similarity in genetic origin and strong gene flow between wild and cultivated compartments. We also confirmed the introduction of Congolese varieties into the SC region where most Robusta coffee production takes place. Identified populations occurred in divergent environmental conditions and 12 environmental variables significantly explained 16.3% of the total allelic variation across populations. The substantial genetic variation within and between Ugandan populations with different climatic envelopes might contain adaptive diversity to cope with climate change. The accessions that we collected have substantially enriched the diversity hosted in the Ugandan collections and thus contribute to ex situ conservation of this vital genetic resource. However, there is an urgent need to develop strategies to enhance complementary in-situ conservation of Coffea canephora in native forests in northwestern Uganda.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Sciences du monde végétal [076]
Description Géographique
OUGANDA
Localisation
Fonds IRD [F B010081077]
Identifiant IRD
fdi:010081077
