Andre S., Raja Z., Humblot V., Piesse C., Foulon T., Sereno Denis, Oury Bruno, Ladram A. (2020). Functional characterization of temporin-she, a new broad-spectrum antibacterial and leishmanicidal temporin-SH paralog from the Sahara frog (Pelophylax saharicus). International Journal of Molecular Sciences, 21 (18), p. 6713 [19 p.].
Titre du document
Functional characterization of temporin-she, a new broad-spectrum antibacterial and leishmanicidal temporin-SH paralog from the Sahara frog (Pelophylax saharicus)
Andre S., Raja Z., Humblot V., Piesse C., Foulon T., Sereno Denis, Oury Bruno, Ladram A.
Source
International Journal of Molecular Sciences, 2020,
21 (18), p. 6713 [19 p.]
Amphibian skin is a promising natural resource for antimicrobial peptides (AMPs), key effectors of innate immunity with attractive therapeutic potential to fight antibiotic-resistant pathogens. Our previous studies showed that the skin of the Sahara Frog (Pelophylax saharicus) contains broad-spectrum AMPs of the temporin family, named temporins-SH. Here, we focused our study on temporin-SHe, a temporin-SHd paralog that we have previously identified in this frog but was never structurally and functionally characterized. We synthesized and determined the structure of temporin-SHe. This non-amphipathic alpha-helical peptide was demonstrated to strongly destabilize the lipid chain packing of anionic multilamellar vesicles mimicking bacterial membranes. Investigation of the antimicrobial activity revealed that temporin-SHe targets Gram-negative and Gram-positive bacteria, including clinical isolates of multi-resistant Staphylococcus aureus strains. Temporin-SHe exhibited also antiparasitic activity toward differentLeishmaniaspecies responsible for visceral leishmaniasis, as well as cutaneous and mucocutaneous forms. Functional assays revealed that temporin-SHe exerts bactericidal effects with membrane depolarization and permeabilization, via a membranolytic mechanism observed by scanning electron microscopy. Temporin-SHe represents a new member of the very limited group of antiparasitic temporins/AMPs. Despite its cytotoxicity, it is nevertheless an interesting tool to study the AMP antiparasitic mechanism and design new antibacterial/antiparasitic agents.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Entomologie médicale / Parasitologie / Virologie [052]
;
Sciences du monde animal [080]
;
Biotechnologies [084]
