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Reusken C., Baronti Cécile, Mogling R., Papa A., Leitmeyer K., Charrel R. N. (2019). Toscana, West Nile, Usutu and tick-borne encephalitis viruses : external quality assessment for molecular detection of emerging neurotropic viruses in Europe, 2017. Eurosurveillance, 24 (50), 18-31. ISSN 1560-7917

Fichier PDF disponiblehttp://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers20-01/010077429.pdf[ PDF Link ]

Lien direct chez l'éditeur doi:10.2807/1560-7917.es.2019.24.50.1900051

Titre
Toscana, West Nile, Usutu and tick-borne encephalitis viruses : external quality assessment for molecular detection of emerging neurotropic viruses in Europe, 2017
Année de publication2019
Type de documentArticle référencé dans le Web of Science WOS:000502554800003
AuteursReusken C., Baronti Cécile, Mogling R., Papa A., Leitmeyer K., Charrel R. N.
SourceEurosurveillance, 2019, 24 (50), p. 18-31. ISSN 1560-7917
RésuméBackground: Neurotropic arboviruses are increasingly recognised as causative agents of neurological disease in Europe but underdiagnosis is still suspected. Capability for accurate diagnosis is a prerequisite for adequate clinical and public health response. Aim: To improve diagnostic capability in EVD-LabNet laboratories, we organised an external quality assessment (EQA) focusing on molecular detection of Toscana (TOSV), Usutu (USUV), West Nile (WNV) and tick-borne encephalitis viruses (TBEV). Methods: Sixty-nine laboratories were invited. The EQA panel included two WNV RNA- positive samples (lineages 1 and 2), two TOSV RNA- positive samples (lineages A and B), one TBEV RNA- positive sample (Western subtype), one USUV RNA- positive sample and four negative samples. The EQA focused on overall capability rather than sensitivity of the used techniques. Only detection of one, clinically relevant, concentration per virus species and lineage was assessed. Results: The final EQA analysis included 51 laboratories from 35 countries; 44 of these laboratories were from 28 of 31 countries in the European Union/European Economic Area (EU/EEA). USUV diagnostic capability was lowest (28 laboratories in 18 countries), WNV detection capacity was highest (48 laboratories in 32 countries). Twenty-five laboratories were able to test the whole EQA panel, of which only 11 provided completely correct results. The highest scores were observed for WNV and TOSV (92%), followed by TBEV (86%) and USUV (75%). Conclusion: We observed wide variety in extraction methods and RT- PCR tests, showing a profound absence of standardisation across European laboratories. Overall, the results were not satisfactory; capacity and capability need to be improved in 40 laboratories.
Plan de classementEntomologie médicale / Parasitologie / Virologie [052] ; Santé : généralités [050]
Descr. géo.EUROPE
LocalisationFonds IRD [F B010077429]
Identifiant IRDfdi:010077429
Lien permanenthttp://www.documentation.ird.fr/hor/fdi:010077429

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