Dekkiche S., Benguedouar A., Sbabou L., Taha K., Filali-Maltouf A., Béna Gilles. (2018). Chickpea (Cicer arietinum) is nodulated by unexpected wide diversity of Mesorhizobium species in Eastern Algeria. Archives of Agronomy and Soil Science, 64 (2), p. 285-297. ISSN 0365-0340.
Titre du document
Chickpea (Cicer arietinum) is nodulated by unexpected wide diversity of Mesorhizobium species in Eastern Algeria
Dekkiche S., Benguedouar A., Sbabou L., Taha K., Filali-Maltouf A., Béna Gilles
Source
Archives of Agronomy and Soil Science, 2018,
64 (2), p. 285-297 ISSN 0365-0340
Using a local Algerian cultivar of chickpea, from 9 sites in Eastern Algeria, along a sub-humid to arid gradient, we isolated 60 nodulating isolates, among which 19 were assigned to Mesorhizobium species and 41 to Ensifer meliloti. Trapping revealed great differences among sites for their ability to induce nodules on plant, but no correlation with chemical and physical characterizations of soil samples could be found. Based on 16S rRNA gene sequences, 16S-23S IGS, recA, nodC and nifH sequences, Mesorhizobium isolates were assigned to at least 5 different species, among which one had never been described as nodulating chickpea before. The symbiotic diversity is conversely low, and shared by several species, reflecting horizontal gene transfer. The ability of Mesorhizobium genus to recombine and exchange symbiotic clusters among different species must play a great role in this ability to be associated with chickpea. The question to what extent the symbiotic species would end in this genus is discussed.
Plan de classement
Pédologie [068]
;
Biologie du sol [074]
;
Biotechnologies [084]
