Publications des scientifiques de l'IRD

Wang L., Rinaldi F. C., Singh P., Doyle E. L., Dubrow Z. E., Tran T. T., Perez-Quintero A. L., Szurek Boris, Bogdanove A. J. (2017). TAL effectors drive transcription bidirectionally in plants. Molecular Plant, 10 (2), p. 285-296. ISSN 1674-2052.

Titre du document
TAL effectors drive transcription bidirectionally in plants
Année de publication
2017
Type de document
Article référencé dans le Web of Science WOS:000397031300006
Auteurs
Wang L., Rinaldi F. C., Singh P., Doyle E. L., Dubrow Z. E., Tran T. T., Perez-Quintero A. L., Szurek Boris, Bogdanove A. J.
Source
Molecular Plant, 2017, 10 (2), p. 285-296 ISSN 1674-2052
TAL effectors delivered by phytopathogenic Xanthomonas species are DNA-sequence-specific transcriptional activators of host susceptibility genes and sometimes resistance genes. The modularity of DNA recognition by TAL effectors makes them important also as tools for gene targeting and genome editing. Effector binding elements (EBEs) recognized by native TAL effectors in plants have been identified only on the forward strand of target promoters. Here, we demonstrate that TAL effectors can drive plant transcription from EBEs on either strand and in both directions. Furthermore, we show that a native TAL effector from Xanthomonas oryzae pv. oryzicola drives expression of a target with an EBE on each strand of its promoter. By inserting that promoter and derivatives between two reporter genes oriented head to head, we show that the TAL effector drives expression from either EBE in the respective orientations, and that activity at the reverse-strand EBE also potentiates forward transcription driven by activity at the forward-strand EBE. Our results reveal new modes of action for TAL effectors, suggesting the possibility of yet unrecognized targets important in plant disease, expanding the search space for off-targets of custom TAL effectors, and highlighting the potential of TAL effectors for probing fundamental aspects of plant transcription.
Plan de classement
Sciences du monde végétal [076]
Localisation
Fonds IRD [F B010069388]
Identifiant IRD
fdi:010069388
Contact