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Dall'Agnol R. F., Plotegher F., Souza R. C., Mendes I. C., dos Reis F. B., Béna Gilles, Moulin Lionel, Hungria M. (2016). Paraburkholderia nodosa is the main N-2-fixing species trapped by promiscuous common bean (Phaseolus vulgaris L.) in the Brazilian 'Cerradao'. Fems Microbiology Ecology, 92 (8), p. fiw108 [14 p.]. ISSN 0168-6496.

Titre du document
Paraburkholderia nodosa is the main N-2-fixing species trapped by promiscuous common bean (Phaseolus vulgaris L.) in the Brazilian 'Cerradao'
Année de publication
2016
Type de document
Article référencé dans le Web of Science WOS:000383230500005
Auteurs
Dall'Agnol R. F., Plotegher F., Souza R. C., Mendes I. C., dos Reis F. B., Béna Gilles, Moulin Lionel, Hungria M.
Source
Fems Microbiology Ecology, 2016, 92 (8), p. fiw108 [14 p.] ISSN 0168-6496
The bacterial genus Burkholderia comprises species occupying several habitats, including a group of symbionts of leguminous plants-also called beta-rhizobia-that has been recently ascribed to the new genus Paraburkholderia. We used common bean (Phaseolus vulgaris L.) plants to trap rhizobia from an undisturbed soil of the Brazilian Cerrado under the vegetation type 'Cerradao'. Genetic characterization started with the analyses of 181 isolates by BOX-PCR, where the majority revealed unique profiles, indicating high inter- and intra-species diversity. Restriction fragment length polymorphism-PCR of the 16S rRNA of representative strains of the BOX-PCR groups indicated two main clusters, and gene-sequencing analysis identified the minority (27%) as Rhizobium and the majority (73%) as Paraburkholderia. Phylogenetic analyses of the 16S rRNA and housekeeping (recA and gyrB) genes positioned all strains of the second cluster in the species P. nodosa, and the phylogeny of a symbiotic gene-nodC-was in agreement with the conserved genes. All isolates were stable vis-A -vis nodulating common bean, but, in general, with a low capacity for fixing N-2, although some effective strains were identified. The predominance of P. nodosa might be associated with the edaphic properties of the Cerrado biome, and might represent an important role in terms of maintenance of the ecosystem, which is characterized by acid soils with high saturation of aluminum and low N-2 content.Study of the diversity of nitrogen-fixing symbionts from a 'hot spot area', the Brazilian 'Cerradao', reveals that Paraburkholderia nodosa is the main symbiont trapped by promiscuous common bean (Phaseolus vulgaris L.).Study of the diversity of nitrogen-fixing symbionts from a 'hot spot area', the Brazilian 'Cerradao', reveals that Paraburkholderia nodosa is the main symbiont trapped by promiscuous common bean (Phaseolus vulgaris L.).
Plan de classement
Biologie du sol [074] ; Etudes, transformation, conservation du milieu naturel [082] ; Biotechnologies [084]
Description Géographique
BRESIL
Localisation
Fonds IRD [F B010068156]
Identifiant IRD
fdi:010068156
Contact