Clavijo F., Diedhiou I., Vaissayre Virginie, Brottier L., Acolatse J., Moukouanga Daniel, Crabos Amandine, Auguy Florence, Franche Claudine, Gherbi Hassen, Champion Antony, Hocher Valérie, Barker D., Bogusz Didier, Tisa L. S., Svistoonoff Sergio. (2015). The Casuarina NIN gene is transcriptionally activated throughout Frankia root infection as well as in response to bacterial diffusible signals. New Phytologist, 208 (3), p. 887-903. ISSN 0028-646X.
Titre du document
The Casuarina NIN gene is transcriptionally activated throughout Frankia root infection as well as in response to bacterial diffusible signals
Année de publication
2015
Auteurs
Clavijo F., Diedhiou I., Vaissayre Virginie, Brottier L., Acolatse J., Moukouanga Daniel, Crabos Amandine, Auguy Florence, Franche Claudine, Gherbi Hassen, Champion Antony, Hocher Valérie, Barker D., Bogusz Didier, Tisa L. S., Svistoonoff Sergio
Source
New Phytologist, 2015,
208 (3), p. 887-903 ISSN 0028-646X
Root nodule symbioses (RNS) allow plants to acquire atmospheric nitrogen by establishing an intimate relationship with either rhizobia, the symbionts of legumes or Frankia in the case of actinorhizal plants. In legumes, NIN (Nodule INception) genes encode key transcription factors involved in nodulation. Here we report the characterization of CgNIN, a NIN gene from the actinorhizal tree Casuarina glauca using both phylogenetic analysis and transgenic plants expressing either ProCgNIN::reporter gene fusions or CgNIN RNAi constructs. We have found that CgNIN belongs to the same phylogenetic group as other symbiotic NIN genes and CgNIN is able to complement a legume nin mutant for the early steps of nodule development. CgNIN expression is correlated with infection by Frankia, including preinfection stages in developing root hairs, and is induced by culture supernatants. Knockdown mutants were impaired for nodulation and early root hair deformation responses were severely affected. However, no mycorrhizal phenotype was observed and no induction of CgNIN expression was detected in mycorrhizas. Our results indicate that elements specifically required for nodulation include NIN and possibly related gene networks derived from the nitrate signalling pathways.
Plan de classement
Sciences du monde végétal [076]
;
Biotechnologies [084]
Localisation
Fonds IRD [F B010065498]
Identifiant IRD
fdi:010065498
