Allahverdiyeva Y., Mustila H., Ermakova M., Bersanini L., Richaud P., Ajlani G., Battchikova N., Cournac Laurent, Aro E. M. (2013). Flavodiiron proteins Flv1 and Flv3 enable cyanobacterial growth and photosynthesis under fluctuating light. Proceedings of the National Academy of Sciences of the United States of America, 110 (10), p. 4111-4116. ISSN 0027-8424.
Titre du document
Flavodiiron proteins Flv1 and Flv3 enable cyanobacterial growth and photosynthesis under fluctuating light
Année de publication
2013
Auteurs
Allahverdiyeva Y., Mustila H., Ermakova M., Bersanini L., Richaud P., Ajlani G., Battchikova N., Cournac Laurent, Aro E. M.
Source
Proceedings of the National Academy of Sciences of the United States of America, 2013,
110 (10), p. 4111-4116 ISSN 0027-8424
Cyanobacterial flavodiiron proteins (FDPs; A-type flavoprotein, Flv) comprise, besides the beta-lactamase-like and flavodoxin domains typical for all FDPs, an extra NAD(P)H:flavin oxidoreductase module and thus differ from FDPs in other Bacteria and Archaea. Synechocystis sp. PCC 6803 has four genes encoding the FDPs. Flv1 and Flv3 function as an NAD(P) H: oxygen oxidoreductase, donating electrons directly to O-2 without production of reactive oxygen species. Here we show that the Flv1 and Flv3 proteins are crucial for cyanobacteria under fluctuating light, a typical light condition in aquatic environments. Under constant-light conditions, regardless of light intensity, the Flv1 and Flv3 proteins are dispensable. In contrast, under fluctuating light conditions, the growth and photosynthesis of the Delta flv1(A) and/or Delta flv3(A) mutants of Synechocystis sp. PCC 6803 and Anabaena sp. PCC 7120 become arrested, resulting in cell death in the most severe cases. This reaction is mainly caused by malfunction of photosystem I and oxidative damage induced by reactive oxygen species generated during abrupt short-term increases in light intensity. Unlike higher plants that lack the FDPs and use the Proton Gradient Regulation 5 to safeguard photosystem I, the cyanobacterial homolog of Proton Gradient Regulation 5 is shown not to be crucial for growth under fluctuating light. Instead, the unique Flv1/Flv3 heterodimer maintains the redox balance of the electron transfer chain in cyanobacteria and provides protection for photosystem I under fluctuating growth light. Evolution of unique cyanobacterial FDPs is discussed as a prerequisite for the development of oxygenic photosynthesis.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Limnologie biologique / Océanographie biologique [034]
Localisation
Fonds IRD [F B010060757]
Identifiant IRD
fdi:010060757
