Liégeois Florian, Boué V., Mouinga-Ondémé A., Lékané D.K., Mongo D., Sica J., Rouet F. (2012). Suitability of an open automated nucleic acid extractor for high-throughput plasma HIV-1 RNA quantitation in Gabon (Central Africa). Journal of Virological Methods, 179 (1), p. 269-271. ISSN 0166-0934.
Titre du document
Suitability of an open automated nucleic acid extractor for high-throughput plasma HIV-1 RNA quantitation in Gabon (Central Africa)
Liégeois Florian, Boué V., Mouinga-Ondémé A., Lékané D.K., Mongo D., Sica J., Rouet F.
Source
Journal of Virological Methods, 2012,
179 (1), p. 269-271 ISSN 0166-0934
Nucleic acid extraction using the open automated EZ1 (Qiagen) instrument, in combination with the Generic HIV Viral Load assay, gave highly concordant HIV-1 RNA viral load results among 181 Gabonese subjects infected with HIV-1, compared to those obtained when performing a manual extraction. Since people living with HIV-1 are being treated with antiretrovirals in Gabon, this automated extraction technique represents an excellent technical method for high-throughput monitoring of HIV-1 RNA viral load.
