Aghokeng Fobang Avelin, Ewane L., Awazi B., Nanfack A., Delaporte Eric, Zekeng L., Peeters Martine. (2005). Enfuvirtide binding domain is highly conserved in non-B HIV type 1 strains from Cameroon, West Central Africa. Aids Research and Human Retroviruses, 21 (5), p. 430-433. ISSN 0889-2229.
Titre du document
Enfuvirtide binding domain is highly conserved in non-B HIV type 1 strains from Cameroon, West Central Africa
Année de publication
2005
Auteurs
Aghokeng Fobang Avelin, Ewane L., Awazi B., Nanfack A., Delaporte Eric, Zekeng L., Peeters Martine
Source
Aids Research and Human Retroviruses, 2005,
21 (5), p. 430-433 ISSN 0889-2229
Recently T-20 or enfuvirtide, the first drug of a new class of antiretrovirals targeting the entry stage of the virus life cycle, has been clinically approved. Enfuvirtide is a peptide derived from the HR2 region of the transmembrane glycoprotein from the HXB2 HIV-1 subtype B prototype strain that binds to the HR1 region. Drug resistance seems to occur in the HR1 region between amino acids 36 and 45. We examined to what extent this region is conserved in 184 non-B strains from Cameroon: 132 (71.7%) CRF02-AG, 14 (7.6%) subtype A, 11 (5.9%) F2, 9 (4.8%) subtype D, 8 (4.3%) subtype G, 4 (2.1%) CRF01-AE, 4 (2.1%) CRF11-cpx, and 2 (1.1%) CRF06-cpx. Among the 184 strains studied, no amino acid mutation was found in the highly conserved three amino acid motif at codons 36-38 (GIV) that are important determinants of viral susceptibility to enfuvirtide. Other common substitutions like Q40H and N42T were also absent. The N42S polymorphism was present in 148 (80.4%) strains. Analysis of the HR2 domain, from which the peptide is derived, indicated a much greater genetic variability as compared to HR1.
Plan de classement
Maladies sexuellement transmissibles [052MALTRA03]
Descripteurs
SIDA ; MEDICAMENT ; TRAITEMENT MEDICAL ; SENSIBILITE RESISTANCE ; VARIABILITE GENETIQUE ; MUTATION ; ANTIRETROVIRAUX ; ENFUVIRTIDE ; ANALYSE GENETIQUE ; DIVERSITE GENETIQUE ; SEQUENCE
Description Géographique
AFRIQUE CENTRALE ; CAMEROUN
Localisation
Fonds IRD [F B010034462] ;
Montpellier (Centre IRD)
Identifiant IRD
fdi:010034462
