Coste J., Montes B., Reynes J., Peeters Martine, Segarra C., Vendrell J.P., Delaporte Eric, Segondy M. (1996). Comparative evaluation of three assays for the quantitation of human immunodeficiency virus type 1 RNA in plasma. Journal of Medical Virology, 50, p. 293-302. ISSN 0146-6615.
Titre du document
Comparative evaluation of three assays for the quantitation of human immunodeficiency virus type 1 RNA in plasma
Année de publication
1996
Auteurs
Coste J., Montes B., Reynes J., Peeters Martine, Segarra C., Vendrell J.P., Delaporte Eric, Segondy M.
Source
Journal of Medical Virology, 1996,
50, p. 293-302 ISSN 0146-6615
Reverse transcriptase-coupled polymerase chain reaction (Amplicor HIV-1 Monitor), the branched DNA (bDNA) method (Quantiplex HIV-1 RNA) and the nucleic acid sequence-based assay (NASBA HIV-1 RNA QT) were comparatively evaluated for the quantitation of human immunodeficiency virus type 1 (HIV-1) RNA in plasma. Among 60 plasma specimens from HIV-1 infected patients, HIV-1 RNA was detected in 56 by Amplicor (sensitivity, 93.3 %), in 41 by bDNA (sensitivity, 68.3 %), and in 60 by NASBA (sensitivity, 100 %). HIV-1 RNA was not detected by any of these methods in 34/34 plasma specimens from HIV-1-seronegative blood donors (specificity, 100 %). The HIV-1 RNA levels as determined by the different methods were correlated significantly. The frequency of concordant results (log difference less than 0.50) was 80.4 % between Amplicor and NASBA, 77.5 % between Amplicor and bDNA, and 58.6 % between bDNA and NASBA. After initiation of antiviral therapy, HIV-1 RNA level variations observed with the three methods were similar. HIV-1 RNA levels were inversely correlated with the CD4+ T cell counts, whereas no correlation was found with HIV-1 p24-antigen levels. When the methods were evaluated for reproducibility, coefficients of variation ranged from 11 % to 40 % for Amplicor, from 6 % to 35 % for bDNA, and from 13 % to 62 % for NASBA. Quantitation of HIV-1 RNA in culture supernatants from HIV-1 subtype A to H strains showed that bDNA can be used to quantitate RNA from all HIV-1 subtypes, whereas Amplicor failed to detect RNA from subtype A strains and NASBA subtype G strains. (Résumé d'auteur)
Plan de classement
Maladies sexuellement transmissibles [052MALTRA03]
Descripteurs
SIDA ; VIRUS ; ARN ; METHODE D'ANALYSE ; ETUDE COMPARATIVE ; VIH-1
Localisation
Fonds IRD [F B010014418]
Identifiant IRD
fdi:010014418
