Christen Pierre, Revah S. (1998). Growth and production of immobilised lipase Rhizopus delemar cultivated in SSF on a synthetic resin (Amberlite). In :
Raimbault Maurice (ed.), Soccol C.R. (ed.), Chuzel G. (ed.). International training course on solid state fermentation. Montpellier : ORSTOM, p. 77-85 multigr. (Document ORSTOM Montpellier ; 98-1). Solid State Fermentation : International Training Course, Curitiba (BRA), 1997/10/06-10.
Titre du document
Growth and production of immobilised lipase Rhizopus delemar cultivated in SSF on a synthetic resin (Amberlite)
Année de publication
1998
Type de document
Colloque
Auteurs
Christen Pierre, Revah S.
In
Raimbault Maurice (ed.), Soccol C.R. (ed.), Chuzel G. (ed.) International training course on solid state fermentation
Solid State Fermentation : International Training Course, Curitiba (BRA), 1997/10/06-10
In this work, a study of lipase production by Rhizopus delemar grown on a polymeric resin (Amberlite IRA 900) added with a medium previously optimised is presented. In the first part, the activity of several commercial preparations and the R. delemar lipase produced in liquid was compared. It also appeared that the resin can adsorb more than 24% of the lipase produced per g Amberlite. Desorption realised using 5g NaCl/g Amberlite at pH 5 allowed to recover 35% of the adsorbed lipase. It was also shown that the resin displayed a thermo-protective effect since no loss in activity was observed when the adsorbed enzyme was heated at 80°C for 24 hours. In solid state fermentation (SSF), the fungus was shown to produce high amounts of enzyme (93 U/g dry Amberlite against only 14 U/ml in submerged culture) when dextrin was used as carbon source after only 24 hours (against 48 hours in liquid culture). Significative activity was also detected with maltose and more surprisingly with glucose (68 and 57 U/g dry Amberlite respectively). The strong inhibitory effect of glucose observed in liquid culture was reduced in SSF. (Résumé d'auteur)
Plan de classement
Fermentations [084FERMEN]
Descripteurs
FERMENTATION EN MILIEU SOLIDE ; PRODUCTION INDUSTRIELLE ; ENZYME ; CHAMPIGNON ; MILIEU DE CULTURE ; LIPASE
