Gutierrez-Lopez R., Egeter B., Paupy Christophe, Rahola Nil, Makanga B., Jiolle D., Bourret V., Melo M., Loiseau C. (2023). Monitoring mosquito richness in an understudied area : can environmental DNA metabarcoding be a complementary approach to adult trapping ?. Bulletin of Entomological Research, [Early access], p. PII S0007485323000147 [ p.]. ISSN 0007-4853.
Titre du document
Monitoring mosquito richness in an understudied area : can environmental DNA metabarcoding be a complementary approach to adult trapping ?
Année de publication
2023
Auteurs
Gutierrez-Lopez R., Egeter B., Paupy Christophe, Rahola Nil, Makanga B., Jiolle D., Bourret V., Melo M., Loiseau C.
Source
Bulletin of Entomological Research, 2023,
[Early access], p. PII S0007485323000147 [ p.] ISSN 0007-4853
Mosquito surveillance programmes are essential to assess the risks of local vector-borne disease outbreaks as well as for early detection of mosquito invasion events. Surveys are usually performed with traditional sampling tools (i.e., ovitraps and dipping method for immature stages or light or decoy traps for adults). Over the past decade, numerous studies have highlighted that environmental DNA (eDNA) sampling can enhance invertebrate species detection and provide community composition metrics. However, the usefulness of eDNA for detection of mosquito species has, to date, been largely neglected. Here, we sampled water from potential larval breeding sites along a gradient of anthropogenic perturbations, from the core of an oil palm plantation to the rainforest on Sao Tome Island (Gulf of Guinea, Africa). We showed that (i) species of mosquitoes could be detected via metabarcoding mostly when larvae were visible, (ii) larvae species richness was greater using eDNA than visual identification and (iii) new mosquito species were also detected by the eDNA approach. We provide a critical discussion of the pros and cons of eDNA metabarcoding for monitoring mosquito species diversity and recommendations for future research directions that could facilitate the adoption of eDNA as a tool for assessing insect vector communities.
Plan de classement
Entomologie médicale / Parasitologie / Virologie [052]
;
Etudes, transformation, conservation du milieu naturel [082]
Description Géographique
GOLFE DE GUINEE ; SAO TOME
Identifiant IRD
PAR00026103
