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Jaafar F.M., Attoui H., Mertens P.P.C., de Micco P., Lamballerie Xavier de. (2005). Identification and functional analysis of VP3, the guanylyltransferase of Banna virus (genus Seadornavirus, family Reoviridae). Journal of General Virology, 86 Part 4, p. 1141-1146. ISSN 0022-1317.

Titre du document
Identification and functional analysis of VP3, the guanylyltransferase of Banna virus (genus Seadornavirus, family Reoviridae)
Année de publication
2005
Type de document
Article référencé dans le Web of Science WOS:000228236300027
Auteurs
Jaafar F.M., Attoui H., Mertens P.P.C., de Micco P., Lamballerie Xavier de
Source
Journal of General Virology, 2005, 86 Part 4, p. 1141-1146 ISSN 0022-1317
Banna virus (BAV) particles contain seven structural proteins: VP4 and VP9 form an outer-capsid layer, whilst the virus core contains three major proteins (VP2, VP8 and VP10) and two minor proteins (VP1 and VP3). Sequence analysis showed that VP3 contains motifs [Kx(I/V/L)S] and (Hx,H) that have previously been identified in the guanylyltransferases of other reoviruses. Incubation of purified BAV-Ch core particles with [alpha-P-32]GTP resulted in exclusive covalent labelling of VP3, demonstrating autoguanylation activity (which is considered indicative of guanylyltransferase activity). Recombinant VP3 prepared in a cell-free expression system was also guanylated under similar reaction conditions, and products were synthesized (in the presence of non-radiolabelled GDP) that co-migrated with GMP, GDP and GpppG during TLC. This reaction, which required magnesium ions for optimum activity, demonstrates that VP3 possesses nucleoside triphosphatase (GTPase) activity and is the BAV guanylyltransferase (RNA 'capping' enzyme).
Plan de classement
Entomologie médicale / Parasitologie / Virologie [052]
Localisation
Fonds IRD [F B010042253]
Identifiant IRD
PAR00000160
Contact