Hamdi O., Ben Hania Wajdi, Postec A., Bouallagui H., Hamdi M., Bonin P., Ollivier Bernard, Fardeau Marie-Laure. (2015). Aminobacterium thunnarium sp nov., a mesophilic, amino acid-degrading bacterium isolated from an anaerobic sludge digester, pertaining to the phylum Synergistetes. International Journal of Systematic and Evolutionary Microbiology, 65 (2), 609-614. ISSN 1466-5026.
Titre du document
Aminobacterium thunnarium sp nov., a mesophilic, amino acid-degrading bacterium isolated from an anaerobic sludge digester, pertaining to the phylum Synergistetes
Année de publication
2015
Auteurs
Hamdi O., Ben Hania Wajdi, Postec A., Bouallagui H., Hamdi M., Bonin P., Ollivier Bernard, Fardeau Marie-Laure
Source
International Journal of Systematic and Evolutionary Microbiology, 2015,
65 (2), 609-614 ISSN 1466-5026
A new Gram-staining-positive, non-sporulating, mesophilic, amino acid-degrading anaerobic bacterium, designated strain OTA 102(T), was isolated from an anaerobic sequencing batch reactor treating wastewater from cooking tuna. The cells were curved rods (0.6-2.5x0.5 mu m) and occurred singly or in pairs. The strain was motile by means of one lateral flagellum. Strain OTA 102(T) grew at temperatures between 30 and 45 degrees C (optimum 40 degrees C), between pH 6.0 and 8.4 (optimum pH 7.2) and NaCl concentrations between 1 and 5% (optimum 2%, w/v). Strain OTA 102(T) required yeast extract for growth. Serine, threonine, glycine, cysteine, citrate, fumarate, alpha-ketoglutarate and pyruvate were fermented. When co-cultured with Methanobacterium formicicum as the hydrogen scavenger, strain OTA 102(T) oxidized alanine, valine, leucine, isoleucine, aspartate, tyrosine, methionine, histidine and asparagine. The genomic DNA G + C content of strain OTA 102(T) was 41.7 mol%. The main fatty acid was iso-C-15:0. Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain OTA 102(T) was related to Aminobacterium colombiense and Aminobacterium mobile (95.5 and 95.2% similarity, respectively), of the phylum Synergistetes. On the basis of phylogenetic, genetic and physiological characteristics, strain OTA 102(T) is proposed to represent a novel species of the genus Aminobacterium, Aminobacterium thunnarium sp. nov. The type strain is OTA 102(T) (=DSM 27500(T)=JCM 19320(T)).
Plan de classement
Biotechnologies [084]
Localisation
Fonds IRD [F B010088409]
Identifiant IRD
fdi:010088409