Ben ali gam Zouhaier, Thioye A., Cayol J. L., Postec A., Bartoli-Joseph M., Vandecasteele C., Erauso G., Labat Marc. (2023). Thermospira aquatica gen. nov., sp. nov., a novel thermophilic spirochete isolated from a Tunisian hot spring, and description of the novel family Thermospiraceae. International Journal of Systematic and Evolutionary Microbiology, 73 (1), [10 p.]. ISSN 1466-5026.
Titre du document
Thermospira aquatica gen. nov., sp. nov., a novel thermophilic spirochete isolated from a Tunisian hot spring, and description of the novel family Thermospiraceae
Année de publication
2023
Auteurs
Ben ali gam Zouhaier, Thioye A., Cayol J. L., Postec A., Bartoli-Joseph M., Vandecasteele C., Erauso G., Labat Marc
Source
International Journal of Systematic and Evolutionary Microbiology, 2023,
73 (1), [10 p.] ISSN 1466-5026
A novel thermophilic, anaerobic bacterium, strain F1F22T, was isolated from hot spring water collected in northern Tunisia. The cells were non-motile, Gram-negative and helical with hooked ends, 0.5x10-32 mu m in size. Growth of the strain was observed at 45-70 degrees C (optimum, 55 degrees C), in 0.0-1.0 % (w/v) NaCl (optimum without NaCl) and at pH 6.5-8.5 (optimum, pH 7.5). Yeast extract was required for growth, and the strain grew on glucose, sucrose and maltose. The major fatty acids were C16:0 (40.2 %), iso- C16: 0 (30.2 %) and C16 :0 DMA (14.5 %). The genome consisted of a circular chromosome (2.5 Mb) containing 2672 predicted protein -encoding genes with a G+C content of 43.15 mol %. Based on a comparative 16S rRNA gene sequence analysis, strain F1F22T formed a deeply branching lineage within the phylum Spirochaetota, class Spirochaetia, order Brevinematales, and had only low sequence similarity to other species of the phylum (lower than 83 %). Genome- based analysis of average nucleotide identity and digital DNA-DNA hybridization of strain F1F22T with Treponema caldarium DSM 7334T, Brevinema andersonii ATCC 43811T and Spirochaeta thermophila DSM 6578T showed values between 63.26 and 63.52 %, and between 20 and 25 %. Hence, we propose strain F1F22T as a representative of a novel family (Thermospiraceae fam. nov.), genus and species of Brevinematales: Ther-mospira aquatica gen. nov., sp. nov. (type strain F1F22T=JCM 31314T=DSM 101182T).
Plan de classement
Limnologie biologique / Océanographie biologique [034]
;
Biotechnologies [084]
Description Géographique
TUNISIE
Localisation
Fonds IRD [F B010088158]
Identifiant IRD
fdi:010088158
