Publications des scientifiques de l'IRD

Moreau X., Claeys-Bruno M., Andraud J. P., Macarie Hervé, Martinez D. E., Robin M., Sergent M., De Jong L. (2022). Hydra bioassay for the evaluation of chlordecone toxicity at environmental concentrations, alone or in complex mixtures with dechlorinated byproducts : experimental observations and modeling by experimental design. Environmental Science and Pollution Research, 29, 91017-91035. ISSN 0944-1344.

Titre du document
Hydra bioassay for the evaluation of chlordecone toxicity at environmental concentrations, alone or in complex mixtures with dechlorinated byproducts : experimental observations and modeling by experimental design
Année de publication
2022
Type de document
Article référencé dans le Web of Science WOS:000830312600004
Auteurs
Moreau X., Claeys-Bruno M., Andraud J. P., Macarie Hervé, Martinez D. E., Robin M., Sergent M., De Jong L.
Source
Environmental Science and Pollution Research, 2022, 29, 91017-91035 ISSN 0944-1344
In chlordecone (CLD)-contaminated soils of the French West Indies, if microbial remediation or a physicochemical remediation process, e.g., in situ chemical reduction, is implemented, concentrations of degradation byproducts, such as hydrochlordecones, are expected to increase in the ecosystems. To study their impact in mixtures with CLD, bioassays were carried out. They consisted in evaluating the regenerative capacity of hydra polyps, from a clone whose phylogenetic analysis confirmed that it belonged to the species Hydra vulgaris Pallas, 1766. Hydra gastric sections were exposed to CLD alone or CLD plus dechlorinated byproducts (CLD-BP) for 96 h to assess regeneration. Based on chromatographic analysis, the CLD-BP mix was composed of the 5-monohydrochlordecone isomer (CAS nomenclature), four dihydrochlordecone isomers, and one trihydrochlordecone isomer representing 50%, 47%, and 3% of the total chromatographic area, respectively. A total of 18 mixtures of CLD and CLD-BP were tested. Six environmental concentrations of CLD (2.10(-4) mu M to 4.10(-2) mu M) and a similar range of CLD-BP were used. Results from exposures to CLD alone showed the following: (i) a significant decrease in the regenerative capacity of hydra, except at the lowest concentration (2.10(-4) mu M); (ii) a concentration-independent deleterious effect. The regeneration scores obtained after the exposure to the addition of CLD-BP were not significantly different from those obtained after exposure to CLD alone. Using an experimental design, a modeling of the regeneration scores of hydra exposed to mixtures is proposed. Interpreted carefully, since they are limited to only one type of bioassay, the present results suggest that the situation in the aquatic environments should not become worse in terms of toxicity, if soil remediation programs resulting in the formation of hydrochlordecones are put in place.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020] ; Sciences du milieu [021] ; Limnologie biologique / Océanographie biologique [034] ; Pollution [038]
Localisation
Fonds IRD [F B010085893]
Identifiant IRD
fdi:010085893
Contact