Bastard J. P., Pelloux V., Alili R., Fellahi S., Aron-Wisnewsky J., Capel E., Feve B., Assoumou L., Prifti Edi, Katlama C., Clement K., Capeau J. (2021). Altered subcutaneous adipose tissue parameters after switching ART-controlled HIV plus patients to raltegravir/maraviroc. AIDS, 35 (10), p. 1625-1630. ISSN 0269-9370.
Titre du document
Altered subcutaneous adipose tissue parameters after switching ART-controlled HIV plus patients to raltegravir/maraviroc
Année de publication
2021
Auteurs
Bastard J. P., Pelloux V., Alili R., Fellahi S., Aron-Wisnewsky J., Capel E., Feve B., Assoumou L., Prifti Edi, Katlama C., Clement K., Capeau J.
Source
AIDS, 2021,
35 (10), p. 1625-1630 ISSN 0269-9370
Objective: To evaluate the effect on anthropometric, metabolic and adipose tissue parameters of switching ART-controlled persons living with HIV (PLWH) from a protease inhibitor regimen to raltegravir/maraviroc. Design: Sub-study of the ANRS157 ROCnRAL study with the investigation of subcutaneous abdominal adipose tissue (SCAT) biopsy at inclusion and study end. Methods: We performed lipoaspiration of paired SCAT samples, histology on fresh/fixed samples and examined the transcriptomic profile analyzed using Illumina microarrays after RNA extraction. Statistical analyses used the Wilcoxon-paired test. Results: The patients (n = 8) were mainly male (7/8), aged (mean +/- standard error of the mean) 54.9 +/- 1.2 years, BMI 26.1 +/- 1.2 kg/m(2), CD4(+) 699 +/- 56 cells/mm(3), all viral load (VL) <50 copies/ml. After a follow-up of 6 +/- 0.5 months, all PLWH remained with VL <50 copies/ml. BMI, trunk and limb fat amounts were unchanged yet systemic insulin resistance increased. Adipose tissue histology was unchanged except for borderline increased adipocyte diameter (P = 0.1). Among the 16 094 RNA transcripts, 458 genes were up-regulated and 244 were down-regulated. Analyses of the Kyoto Encyclopedia of Genes and Genomes and Gene Ontology databases, evaluating modifications in the main functional pathways, revealed that genes related to immune recognition/function were less expressed as were genes encoding T-cell receptor and receptor signaling pathways. The gene expression profiles indicated decreased inflammation but genes involved in adipogenesis and insulin resistance were overexpressed. Conclusion: After 6 months of raltegravir/maraviroc, adipogenesis-related gene profile was enhanced in SCAT, in agreement with a tendency for increased adipocyte size. Enhanced SCAT insulin resistance-related profile was concordant with higher systemic insulin resistance. However, the immune activation/inflammation profile was globally lowered. We propose that raltegravir/maraviroc might favor SCAT gain but reduce inflammation/immune activation.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Santé : généralités [050]
;
Entomologie médicale / Parasitologie / Virologie [052]
Description Géographique
FRANCE
Localisation
Fonds IRD [F B010083247]
Identifiant IRD
fdi:010083247
