%0 Journal Article
%9 ACL : Articles dans des revues avec comité de lecture répertoriées par l'AERES
%A Bastard, J. P.
%A Pelloux, V.
%A Alili, R.
%A Fellahi, S.
%A Aron-Wisnewsky, J.
%A Capel, E.
%A Feve, B.
%A Assoumou, L.
%A Prifti, Edi
%A Katlama, C.
%A Clement, K.
%A Capeau, J.
%T Altered subcutaneous adipose tissue parameters after switching ART-controlled HIV plus patients to raltegravir/maraviroc
%D 2021
%L fdi:010083247
%G ENG
%J AIDS
%@ 0269-9370
%K adipogenesis ; histology ; inflammation ; immune activation ; insulin ; resistance ; maraviroc ; raltegravir ; subcutaneous adipose tissue ; transcriptomics
%K FRANCE
%M ISI:000708514200011
%N 10
%P 1625-1630
%R 10.1097/qad.0000000000002900
%U https://www.documentation.ird.fr/hor/fdi:010083247
%> https://www.documentation.ird.fr/intranet/publi/2021-11/010083247.pdf
%V 35
%W Horizon (IRD)
%X Objective: To evaluate the effect on anthropometric, metabolic and adipose tissue parameters of switching ART-controlled persons living with HIV (PLWH) from a protease inhibitor regimen to raltegravir/maraviroc. Design: Sub-study of the ANRS157 ROCnRAL study with the investigation of subcutaneous abdominal adipose tissue (SCAT) biopsy at inclusion and study end. Methods: We performed lipoaspiration of paired SCAT samples, histology on fresh/fixed samples and examined the transcriptomic profile analyzed using Illumina microarrays after RNA extraction. Statistical analyses used the Wilcoxon-paired test. Results: The patients (n = 8) were mainly male (7/8), aged (mean +/- standard error of the mean) 54.9 +/- 1.2 years, BMI 26.1 +/- 1.2 kg/m(2), CD4(+) 699 +/- 56 cells/mm(3), all viral load (VL) <50 copies/ml. After a follow-up of 6 +/- 0.5 months, all PLWH remained with VL <50 copies/ml. BMI, trunk and limb fat amounts were unchanged yet systemic insulin resistance increased. Adipose tissue histology was unchanged except for borderline increased adipocyte diameter (P = 0.1). Among the 16 094 RNA transcripts, 458 genes were up-regulated and 244 were down-regulated. Analyses of the Kyoto Encyclopedia of Genes and Genomes and Gene Ontology databases, evaluating modifications in the main functional pathways, revealed that genes related to immune recognition/function were less expressed as were genes encoding T-cell receptor and receptor signaling pathways. The gene expression profiles indicated decreased inflammation but genes involved in adipogenesis and insulin resistance were overexpressed. Conclusion: After 6 months of raltegravir/maraviroc, adipogenesis-related gene profile was enhanced in SCAT, in agreement with a tendency for increased adipocyte size. Enhanced SCAT insulin resistance-related profile was concordant with higher systemic insulin resistance. However, the immune activation/inflammation profile was globally lowered. We propose that raltegravir/maraviroc might favor SCAT gain but reduce inflammation/immune activation.
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