Sow M.D., Le Gac A.L., Fichot R., Lanciano S., Delaunay A., Le Jan I., Lesage-Descauses M.C., Citerne S., Caius J., Brunaud V., Soubigou-Taconnat L., Cochard H., Segura V., Chaparro C., Grunau C., Daviaud C., Tost J., Brignolas F., Strauss S.H., Mirouze Marie, Maury S. (2021). RNAi suppression of DNA methylation affects the drought stress response and genome integrity in transgenic poplar. New Phytologist, 232 (1), 80-97. ISSN 0028-646X.
Titre du document
RNAi suppression of DNA methylation affects the drought stress response and genome integrity in transgenic poplar
Année de publication
2021
Auteurs
Sow M.D., Le Gac A.L., Fichot R., Lanciano S., Delaunay A., Le Jan I., Lesage-Descauses M.C., Citerne S., Caius J., Brunaud V., Soubigou-Taconnat L., Cochard H., Segura V., Chaparro C., Grunau C., Daviaud C., Tost J., Brignolas F., Strauss S.H., Mirouze Marie, Maury S.
Source
New Phytologist, 2021,
232 (1), 80-97 ISSN 0028-646X
Trees are long-lived organisms that continuously adapt to their environments, a process in which epigenetic mechanisms are likely to play a key role. Via down regulation of the chromatin remodeler DECREASED IN DNA METHYLATION 1 (DDM1) in poplar (Populus tremula 9 Populus alba) RNAi lines, we examined how DNA methylation coordinates genomic and physiological responses to moderate water deficit.We compared the growth and drought response of two RNAi-ddm1 lines to wild-type (WT) trees under well-watered and water deficit/rewatering conditions, and analyzed their methylomes, transcriptomes, mobilomes and phytohormone contents in the shoot apical meristem. The RNAi-ddm1 lines were more tolerant to drought-induced cavitation but did not differ in height or stem diameter growth. About 5000 differentially methylated regions were consistently detected in both RNAi-ddm1 lines, colocalizing with 910 genes and 89 active transposable elements. Under water deficit conditions, 136 differentially expressed genes were found, including many involved in phytohormone pathways ; changes in phytohormone concentrations were also detected. Finally, the combination of hypomethylation and drought led to the mobility of two transposable elements. Our findings suggest major roles for DNA methylation in regulation of genes involved in hormone-related stress responses, and the maintenance of genome integrity through repression of transposable elements.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Sciences du monde végétal [076]
;
Etudes, transformation, conservation du milieu naturel [082]
Localisation
Fonds IRD [F B010082448]
Identifiant IRD
fdi:010082448
