@article{fdi:010082448,
  title = {{RNA}i suppression of {DNA} methylation affects the drought stress response and genome integrity in transgenic poplar},
  author = {{S}ow, {M}.{D}. and {L}e {G}ac, {A}.{L}. and {F}ichot, {R}. and {L}anciano, {S}. and {D}elaunay, {A}. and {L}e {J}an, {I}. and {L}esage-{D}escauses, {M}.{C}. and {C}iterne, {S}. and {C}aius, {J}. and {B}runaud, {V}. and {S}oubigou-{T}aconnat, {L}. and {C}ochard, {H}. and {S}egura, {V}. and {C}haparro, {C}. and {G}runau, {C}. and {D}aviaud, {C}. and {T}ost, {J}. and {B}rignolas, {F}. and {S}trauss, {S}.{H}. and {M}irouze, {M}arie and {M}aury, {S}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}rees are long-lived organisms that continuously adapt to their environments, a process in which epigenetic mechanisms are likely to play a key role. {V}ia down regulation of the chromatin remodeler {DECREASED} {IN} {DNA} {METHYLATION} 1 ({DDM}1) in poplar ({P}opulus tremula 9 {P}opulus alba) {RNA}i lines, we examined how {DNA} methylation coordinates genomic and physiological responses to moderate water deficit.{W}e compared the growth and drought response of two {RNA}i-ddm1 lines to wild-type ({WT}) trees under well-watered and water deficit/rewatering conditions, and analyzed their methylomes, transcriptomes, mobilomes and phytohormone contents in the shoot apical meristem. {T}he {RNA}i-ddm1 lines were more tolerant to drought-induced cavitation but did not differ in height or stem diameter growth. {A}bout 5000 differentially methylated regions were consistently detected in both {RNA}i-ddm1 lines, colocalizing with 910 genes and 89 active transposable elements. {U}nder water deficit conditions, 136 differentially expressed genes were found, including many involved in phytohormone pathways ; changes in phytohormone concentrations were also detected. {F}inally, the combination of hypomethylation and drought led to the mobility of two transposable elements. {O}ur findings suggest major roles for {DNA} methylation in regulation of genes involved in hormone-related stress responses, and the maintenance of genome integrity through repression of transposable elements.},
  keywords = {},
  booktitle = {},
  journal = {{N}ew {P}hytologist},
  volume = {232},
  numero = {1},
  pages = {80--97},
  ISSN = {0028-646{X}},
  year = {2021},
  DOI = {10.1111/nph.17555},
  URL = {https://www.documentation.ird.fr/hor/fdi:010082448},
}