Serological biomarker for assessing human exposure to Aedes mosquito bites during a randomized vector control intervention trial in northeastern Thailand

2021

Article référencé dans le Web of Science WOS:000664506600004

Fustec Bénédicte, Phanitchat T., Aromseree S., Pientong C., Thaewnongiew K., Ekalaksananan T., Cerqueira D., Poinsignon Anne, Elguero Eric, Bangs M. J., Alexander N., Overgaard H. J., Corbel Vincent

PLoS Neglected Tropical Diseases, 2021, 15 (5), e0009440 [22 p.] ISSN 1935-2735

Background Aedes mosquitoes are vectors for several major arboviruses of public health concern including dengue viruses. The relationships between Aedes infestation and disease transmission are complex wherein the epidemiological dynamics can be difficult to discern because of a lack of robust and sensitive indicators for predicting transmission risk. This study investigates the use of anti-Aedes saliva antibodies as a serological biomarker for Aedes mosquito bites to assess small scale variations in adult Aedes density and dengue virus (DENV) transmission risk in northeastern Thailand. Individual characteristics, behaviors/occupation and socio-demographics, climatic and epidemiological risk factors associated with human-mosquito exposure are also addressed. Methods The study was conducted within a randomized clustered control trial in Roi Et and Khon Kaen provinces over a consecutive 19 months period. Thirty-six (36) clusters were selected, each of ten houses. Serological and entomological surveys were conducted in all houses every four months and monthly in three sentinel households per cluster between September 2017 and April 2019 for blood spot collections and recording concurrent immature and adult Aedes indices. Additionally, the human exposure to Aedes mosquito bites (i.e., Mosquito Exposure Index or MEI) was estimated by ELISA measuring levels of human antibody response to the specific Nterm-34 kDa salivary antigen. The relationships between the MEI, vector infestation indices (adult and immature stages) and vector DENV infection were evaluated using a two-level (house and individual levels) mixed model analysis with one-month lag autoregressive correlation. Results There was a strong positive relationship between the MEI and adult Aedes (indoor and outdoor) density. Individuals from households with a medium mosquito density (mean difference: 0.091, p<0.001) and households with a high mosquito density (mean difference: 0.131, p<0.001) had higher MEI's compared to individuals from households without Aedes. On a similar trend, individuals from households with a low, medium or high indoor Aedes densities (mean difference: 0.021, p<0.007, 0.053, p<0.0001 and 0.037, p<0.0001 for low, medium and high levels of infestation, respectively) had higher MEI than individuals from houses without indoor Aedes., The MEI was driven by individual characteristics, such as gender, age and occupation/behaviors, and varied according to climatic, seasonal factors and vector control intervention (p<0.05). Nevertheless, the study did not demonstrate a clear correlation between MEI and the presence of DENV-infected Aedes. Conclusion This study represents an important step toward the validation of the specific IgG response to the Aedes salivary peptide Nterm-34kDa as a proxy measure for Aedes infestation levels and human-mosquito exposure risk in a dengue endemic setting. The use of the IgG response to the Nterm-34 kDa peptide as a viable diagnostic tool for estimating dengue transmission requires further investigations and validation in other geographical and transmission settings. Author summaryAedes mosquitoes and the viruses they transmit are major public health concerns for over half of the global human population. However, the quantitative relationships between virus transmission and vector mosquito infestation remain unclear despite numerous indicators used to estimate transmission risk and predict dengue outbreaks. The aim of this study is to investigate the use of a salivary biomarker to assess the small-scale variation in human exposure to Aedes bites and the risk of dengue infection in the context of a vector control intervention in northeastern Thailand. A cohort of 539 persons visited every four months, including 161 individuals visited monthly, were recruited for routine serological and concurrent household entomological surveys during 19 consecutive months follow-up. Antibody response to Aedes bites was measured by enzyme-linked immunosorbent assays to assess the mosquito exposure index (MEI) and association with the Aedes adult and immature abundance as well as the presence of dengue virus (DENV) in adult mosquitoes (transmission risk). Additionally, the individual (cohort), climatic, and vector control intervention risk factors associated with MEI are explored. This study demonstrates that the MEI was strongly related to household adult Aedes density, particularly indoors resting mosquitoes. Additionally, the MEI was influenced by individual characteristics (i.e., person age, gender, staying indoors), and varied according to seasons and intervention. Nonetheless, no clear relationship between MEI and dengue transmission risk (i.e., vector infection) was detected. This study demonstrated the potential usefulness of the MEI to assess heterogeneity in adult Aedes infestation indices that could assist public health authorities to rapidly identify mosquito "hot spots" and the timeliness of effective vector control interventions.

Santé : généralités [050] ; Entomologie médicale / Parasitologie / Virologie [052]

THAILANDE

Fonds IRD [F B010082286]

fdi:010082286