Duong Benoit, Nguyen H. X., Phan H. V., Colella S., Trinh P. Q., Hoang G. T., Nguyen T. T., Marraccini P., Lebrun Michel, Duponnois Robin. (2021). Identification and characterization of Vietnamese coffee bacterial endophytes displaying in vitro antifungal and nematicidal activities. Microbiological Research, 242, 126613 [13 p.}. ISSN 0944-5013.
Titre du document
Identification and characterization of Vietnamese coffee bacterial endophytes displaying in vitro antifungal and nematicidal activities
Année de publication
2021
Auteurs
Duong Benoit, Nguyen H. X., Phan H. V., Colella S., Trinh P. Q., Hoang G. T., Nguyen T. T., Marraccini P., Lebrun Michel, Duponnois Robin
Source
Microbiological Research, 2021,
242, 126613 [13 p.} ISSN 0944-5013
The endophytic bacteria were isolated from coffee roots and seeds in Vietnam and identified with 16S rDNA sequencing as belonging to the Actinobacteria, Firmicutes and Proteobacteria phyla with the Nocardia, Bacillus and Burkholderia as dominant genera, respectively. Out of the thirty genera recovered from Coffea canephora and Coffea liberica, twelve were reported for the first time in endophytic association with coffee including members of the genera Brachybacterium, Caballeronia, Kitasatospora, Lechevalieria, Leifsonia, Luteibacter, Lysinibacillus, Mycolicibacterium, Nakamurella, Paracoccus, Sinomonas and Sphingobium. A total of eighty bacterial endophytes were characterized in vitro for several plant growth promoting and biocontrol traits including: the phosphate solubilization, the indolic compounds, siderophores, HCN, esterase, lipase, gelatinase and chitinase production. A subset of fifty selected bacteria were tested for their potential as biocontrol agents with in vitro confrontations with the fungal pathogen Fusarium oxysporum as well as the coffee parasitic nematodes Radopholus duriophilus and Pratylenchus coffeae. The three most efficient isolates on F. oxysporum belonging to the Bacillus, Burkholderia, and Streptomyces genera displayed a growth inhibition rate higher than 40%. Finally, five isolates from the Bacillus genus were able to lead to 100% of mortality in 24 h on both R. duriophilus and P. coffeae.
Plan de classement
Sciences du monde végétal [076]
;
Biotechnologies [084]
Description Géographique
VIET NAM
Localisation
Fonds IRD [F B010080549]
Identifiant IRD
fdi:010080549
