Chu D.K.W., Hui K.P.Y., Perera R.A.P.M., Miguel Eve, Niemeyer D., Zhao J., Channappanavar R., Dudas G., Oladipo J.O., Traoré A., Fassi-Fihri O., Ali A., Demissié G.F., Muth D., Chan M.C.W., Nicholls J.M., Meyerholz D.K., Kuranga S.A., Mamo G., Zhou Z., So R.T.Y., Hemida M.G., Webby R.J., Roger F., Rambaut A., Poon L.L.M., Perlman S., Drosten C., Chevalier V., Peiris M. (2018). MERS coronaviruses from camels in Africa exhibit region-dependent genetic diversity. Proceedings of the National Academy of Sciences of the United States of America, 115 (12), p. 3144-3149. ISSN 0027-8424.
Titre du document
MERS coronaviruses from camels in Africa exhibit region-dependent genetic diversity
Année de publication
2018
Auteurs
Chu D.K.W., Hui K.P.Y., Perera R.A.P.M., Miguel Eve, Niemeyer D., Zhao J., Channappanavar R., Dudas G., Oladipo J.O., Traoré A., Fassi-Fihri O., Ali A., Demissié G.F., Muth D., Chan M.C.W., Nicholls J.M., Meyerholz D.K., Kuranga S.A., Mamo G., Zhou Z., So R.T.Y., Hemida M.G., Webby R.J., Roger F., Rambaut A., Poon L.L.M., Perlman S., Drosten C., Chevalier V., Peiris M.
Source
Proceedings of the National Academy of Sciences of the United States of America, 2018,
115 (12), p. 3144-3149 ISSN 0027-8424
Middle East respiratory syndrome coronavirus (MERS-CoV) causes azoonotic respiratory disease of global public health concern, and dromedary camels are the only proven source of zoonotic infection.Although MERS-CoV infection is ubiquitous in dromedaries across Africaas well as in the Arabian Peninsula, zoonotic disease appears confined tothe Arabian Peninsula. MERS-CoVs from Africa have hitherto beenpoorly studied. We genetically and phenotypically characterized MERS-CoV from dromedaries sampled in Morocco, Burkina Faso, Nigeria, and Ethiopia. Viruses from Africa (clade C) are phylogenetically distinct from contemporary viruses from the Arabian Peninsula (clades A and B) but remain antigenically similar in microneutralization tests. Viruses from West (Nigeria, Burkina Faso) and North (Morocco) Africa form a subclade, C1, that shares clade-defining genetic signatures including deletions in the accessory gene ORF4b.Compared with human and came l MERS-CoV from Saudi Arabia, virus isolates from Burkina Faso (BF785) and Nigeria (Nig1657) had lower virus replication compe-tence in Calu-3 cells and in ex vivo cultures of human bronchus and lung. BF785 replicated to lower titer in lungs of human DPP4-transduced mice. A reverse genetics-derived recombinant MERS-CoV(EMC) lacking ORF4 belicited higher type I and III IFN responses than the isogenic EMC virus in Calu-3 cells. However,ORF4b deletions may not be the major determinant of the reduced replication competence of BF785 and Nig1657. Genetic and phenotypic differences in West African viruses may be relevant to zoonotic potential. There is an urgent need for studies of MERS-CoV at the animal–human interface.
Plan de classement
Santé : généralités [050]
;
Entomologie médicale / Parasitologie / Virologie [052]
;
Sciences du monde animal [080]
Localisation
Fonds IRD [F B010079246]
Identifiant IRD
fdi:010079246
