@article{fdi:010079246,
  title = {{MERS} coronaviruses from camels in {A}frica exhibit region-dependent genetic diversity},
  author = {{C}hu, {D}.{K}.{W}. and {H}ui, {K}.{P}.{Y}. and {P}erera, {R}.{A}.{P}.{M}. and {M}iguel, {E}ve and {N}iemeyer, {D}. and {Z}hao, {J}. and {C}hannappanavar, {R}. and {D}udas, {G}. and {O}ladipo , {J}.{O}. and {T}raor{\'e}, {A}. and {F}assi-{F}ihri, {O}. and {A}li, {A}. and {D}emissi{\'e}, {G}.{F}. and {M}uth, {D}. and {C}han, {M}.{C}.{W}. and {N}icholls, {J}.{M}. and {M}eyerholz, {D}.{K}. and {K}uranga, {S}.{A}. and {M}amo, {G}. and {Z}hou, {Z}. and {S}o, {R}.{T}.{Y}. and {H}emida, {M}.{G}. and {W}ebby, {R}.{J}. and {R}oger, {F}. and {R}ambaut, {A}. and {P}oon, {L}.{L}.{M}. and {P}erlman, {S}. and {D}rosten, {C}. and {C}hevalier, {V}. and {P}eiris, {M}.},
  editor = {},
  language = {{ENG}},
  abstract = {{M}iddle {E}ast respiratory syndrome coronavirus ({MERS}-{C}o{V}) causes azoonotic respiratory disease of global public health concern, and dromedary camels are the only proven source of zoonotic infection.{A}lthough {MERS}-{C}o{V} infection is ubiquitous in dromedaries across {A}fricaas well as in the {A}rabian {P}eninsula, zoonotic disease appears confined tothe {A}rabian {P}eninsula. {MERS}-{C}o{V}s from {A}frica have hitherto beenpoorly studied. {W}e genetically and phenotypically characterized {MERS}-{C}o{V} from dromedaries sampled in {M}orocco, {B}urkina {F}aso, {N}igeria, and {E}thiopia. {V}iruses from {A}frica (clade {C}) are phylogenetically distinct from contemporary viruses from the {A}rabian {P}eninsula (clades {A} and {B}) but remain antigenically similar in microneutralization tests. {V}iruses from {W}est ({N}igeria, {B}urkina {F}aso) and {N}orth ({M}orocco) {A}frica form a subclade, {C}1, that shares clade-defining genetic signatures including deletions in the accessory gene {ORF}4b.{C}ompared with human and came l {MERS}-{C}o{V} from {S}audi {A}rabia, virus isolates from {B}urkina {F}aso ({BF}785) and {N}igeria ({N}ig1657) had lower virus replication compe-tence in {C}alu-3 cells and in ex vivo cultures of human bronchus and lung. {BF}785 replicated to lower titer in lungs of human {DPP}4-transduced mice. {A} reverse genetics-derived recombinant {MERS}-{C}o{V}({EMC}) lacking {ORF}4 belicited higher type {I} and {III} {IFN} responses than the isogenic {EMC} virus in {C}alu-3 cells. {H}owever,{ORF}4b deletions may not be the major determinant of the reduced replication competence of {BF}785 and {N}ig1657. {G}enetic and phenotypic differences in {W}est {A}frican viruses may be relevant to zoonotic potential. {T}here is an urgent need for studies of {MERS}-{C}o{V} at the animal–human interface.},
  keywords = {{MOYEN} {ORIENT} ; {AFRIQUE} {DE} {L}'{OUEST} ; {MAROC} ; {BURKINA} {FASO} ; {NIGERIA} ; {ETHIOPIE}},
  booktitle = {},
  journal = {{P}roceedings of the {N}ational {A}cademy of {S}ciences of the {U}nited {S}tates of {A}merica},
  volume = {115},
  numero = {12},
  pages = {3144--3149},
  ISSN = {0027-8424},
  year = {2018},
  DOI = {10.1073/pnas.1718769115},
  URL = {https://www.documentation.ird.fr/hor/fdi:010079246},
}