Mancini M., Permingeat H., Colono C., Siena L., Pupilli F., Azzaro C., Dusi D. M. D., Carneiro V. T. D., Podio M., Seijo J. G., Gonzalez A. M., Felitti S. A., Ortiz J. P. A., Leblanc Olivier, Pessino S. C. (2018). The MAP3K-coding QUI-GON JINN (QGJ) gene is essential to the formation of unreduced embryo sacs in Paspalum. Frontiers in Plant Science, 9, p. art. 1547 [15 p.]. ISSN 1664-462X.
Titre du document
The MAP3K-coding QUI-GON JINN (QGJ) gene is essential to the formation of unreduced embryo sacs in Paspalum
Année de publication
2018
Auteurs
Mancini M., Permingeat H., Colono C., Siena L., Pupilli F., Azzaro C., Dusi D. M. D., Carneiro V. T. D., Podio M., Seijo J. G., Gonzalez A. M., Felitti S. A., Ortiz J. P. A., Leblanc Olivier, Pessino S. C.
Source
Frontiers in Plant Science, 2018,
9, p. art. 1547 [15 p.] ISSN 1664-462X
Apomixis is a clonal mode of reproduction via seeds, which results from the failure of meiosis and fertilization in the sexual female reproductive pathway. In previous transcriptomic surveys, we identified a mitogen-activated protein kinase kinase kinase (N46) displaying differential representation in florets of sexual and apomictic Paspalum notatum genotypes. Here, we retrieved and characterized the N46 full cDNA sequence from sexual and apomictic floral transcriptomes. Phylogenetic analyses showed that N46 was a member of the YODA family, which was re-named QUI-GON JINN (QGJ). Differential expression in florets of sexual and apomictic plants was confirmed by qPCR. In situ hybridization experiments revealed expression in the nucellus of aposporous plants' ovules, which was absent in sexual plants. RNAi inhibition of QGJ expression in two apomictic genotypes resulted in significantly reduced rates of aposporous embryo sac formation, with respect to the level detected in wild type aposporous plants and transformation controls. The QGJ locus segregated independently of apospory. However, a probe derived from a related long non-coding RNA sequence (PN_LNC_QGJ) revealed RFLP bands cosegregating with the Paspalum apospory-controlling region (ACR). PN_LNC_QGJ is expressed in florets of apomictic plants only. Our results indicate that the activity of QGJ in the nucellus of apomictic plants is necessary to form non-reduced embryo sacs and that a long non-coding sequence with regulatory potential is similar to sequences located within the ACR.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Sciences du monde végétal [076]
Localisation
Fonds IRD [F B010074165]
Identifiant IRD
fdi:010074165
