@article{fdi:010074165,
  title = {{T}he {MAP}3{K}-coding {QUI}-{GON} {JINN} ({QGJ}) gene is essential to the formation of unreduced embryo sacs in {P}aspalum},
  author = {{M}ancini, {M}. and {P}ermingeat, {H}. and {C}olono, {C}. and {S}iena, {L}. and {P}upilli, {F}. and {A}zzaro, {C}. and {D}usi, {D}. {M}. {D}. and {C}arneiro, {V}. {T}. {D}. and {P}odio, {M}. and {S}eijo, {J}. {G}. and {G}onzalez, {A}. {M}. and {F}elitti, {S}. {A}. and {O}rtiz, {J}. {P}. {A}. and {L}eblanc, {O}livier and {P}essino, {S}. {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{A}pomixis is a clonal mode of reproduction via seeds, which results from the failure of meiosis and fertilization in the sexual female reproductive pathway. {I}n previous transcriptomic surveys, we identified a mitogen-activated protein kinase kinase kinase ({N}46) displaying differential representation in florets of sexual and apomictic {P}aspalum notatum genotypes. {H}ere, we retrieved and characterized the {N}46 full c{DNA} sequence from sexual and apomictic floral transcriptomes. {P}hylogenetic analyses showed that {N}46 was a member of the {YODA} family, which was re-named {QUI}-{GON} {JINN} ({QGJ}). {D}ifferential expression in florets of sexual and apomictic plants was confirmed by q{PCR}. {I}n situ hybridization experiments revealed expression in the nucellus of aposporous plants' ovules, which was absent in sexual plants. {RNA}i inhibition of {QGJ} expression in two apomictic genotypes resulted in significantly reduced rates of aposporous embryo sac formation, with respect to the level detected in wild type aposporous plants and transformation controls. {T}he {QGJ} locus segregated independently of apospory. {H}owever, a probe derived from a related long non-coding {RNA} sequence ({PN}_{LNC}_{QGJ}) revealed {RFLP} bands cosegregating with the {P}aspalum apospory-controlling region ({ACR}). {PN}_{LNC}_{QGJ} is expressed in florets of apomictic plants only. {O}ur results indicate that the activity of {QGJ} in the nucellus of apomictic plants is necessary to form non-reduced embryo sacs and that a long non-coding sequence with regulatory potential is similar to sequences located within the {ACR}.},
  keywords = {apomixis ; apospory ; {LNC}-{QGJ} ; {MAP}3{K} ; {P}aspalum notatum ; plant reproduction ; {QGJ} ; {QUI}-{GON}},
  booktitle = {},
  journal = {{F}rontiers in {P}lant {S}cience},
  volume = {9},
  numero = {},
  pages = {art. 1547 [15 p.]},
  ISSN = {1664-462{X}},
  year = {2018},
  DOI = {10.3389/fpls.2018.01547},
  URL = {https://www.documentation.ird.fr/hor/fdi:010074165},
}