Di Lorenzo F., Palmigiano A., Duda K. A., Pallach M., Busset N., Sturiale L., Giraud Eric, Garozzo D., Molinaro A., Silipo A. (2017). Structure of the lipopolysaccharide from the Bradyrhizobium sp ORS285 rfaL mutant strain. Chemistryopen, 6 (4), p. 541-553. ISSN 2191-1363.
Titre du document
Structure of the lipopolysaccharide from the Bradyrhizobium sp ORS285 rfaL mutant strain
Année de publication
2017
Auteurs
Di Lorenzo F., Palmigiano A., Duda K. A., Pallach M., Busset N., Sturiale L., Giraud Eric, Garozzo D., Molinaro A., Silipo A.
Source
Chemistryopen, 2017,
6 (4), p. 541-553 ISSN 2191-1363
The importance of the outer membrane and of its main constituent, lipopolysaccharide, in the symbiosis between rhizobia and leguminous host plants has been well studied. Here, the first complete structural characterization of the entire lipopolysaccharide from an O-chain-deficient Bradyrhizobium ORS285 rfaL mutant is achieved by a combination of chemical analysis, NMR spectroscopy, MALDI MS and MS/MS. The lipidA structure is shown to be consistent with previously reported Bradyrhizobium lipidA, that is, a heterogeneous blend of penta- to hepta-acylated species carrying a nonstoichiometric hopanoid unit and possessing very-long-chain fatty acids ranging from 26:0(25-OH) to 32:0(31-OH). The structure of the core oligosaccharide region, fully characterized for the first time here, is revealed to be a nonphosphorylated linear chain with methylated sugar residues, with a heptose residue exclusively present in the outer core region, and with the presence of two singly substituted 3-deoxy-d-manno-oct-2-ulosonic acid (Kdo) residues, one of which is located in the outer core region. The lipidA moiety is linked to the core moiety through an uncommon 4-substituted Kdo unit.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Biotechnologies [084]
Localisation
Fonds IRD [F B010070865]
Identifiant IRD
fdi:010070865
