@article{fdi:010070865,
  title = {{S}tructure of the lipopolysaccharide from the {B}radyrhizobium sp {ORS}285 rfa{L} mutant strain},
  author = {{D}i {L}orenzo, {F}. and {P}almigiano, {A}. and {D}uda, {K}. {A}. and {P}allach, {M}. and {B}usset, {N}. and {S}turiale, {L}. and {G}iraud, {E}ric and {G}arozzo, {D}. and {M}olinaro, {A}. and {S}ilipo, {A}.},
  editor = {},
  language = {{ENG}},
  abstract = {{T}he importance of the outer membrane and of its main constituent, lipopolysaccharide, in the symbiosis between rhizobia and leguminous host plants has been well studied. {H}ere, the first complete structural characterization of the entire lipopolysaccharide from an {O}-chain-deficient {B}radyrhizobium {ORS}285 rfa{L} mutant is achieved by a combination of chemical analysis, {NMR} spectroscopy, {MALDI} {MS} and {MS}/{MS}. {T}he lipid{A} structure is shown to be consistent with previously reported {B}radyrhizobium lipid{A}, that is, a heterogeneous blend of penta- to hepta-acylated species carrying a nonstoichiometric hopanoid unit and possessing very-long-chain fatty acids ranging from 26:0(25-{OH}) to 32:0(31-{OH}). {T}he structure of the core oligosaccharide region, fully characterized for the first time here, is revealed to be a nonphosphorylated linear chain with methylated sugar residues, with a heptose residue exclusively present in the outer core region, and with the presence of two singly substituted 3-deoxy-d-manno-oct-2-ulosonic acid ({K}do) residues, one of which is located in the outer core region. {T}he lipid{A} moiety is linked to the core moiety through an uncommon 4-substituted {K}do unit.},
  keywords = {bradyrhizobium {ORS}285 ; lipooligosaccharides ; mass spectrometry ; {NMR} ; spectroscopy ; symbiosis},
  booktitle = {},
  journal = {{C}hemistryopen},
  volume = {6},
  numero = {4},
  pages = {541--553},
  ISSN = {2191-1363},
  year = {2017},
  DOI = {10.1002/open.201700074},
  URL = {https://www.documentation.ird.fr/hor/fdi:010070865},
}