Faucon F., Gaude T., Dusfour I., Navratil V., Corbel Vincent, Juntarajumnong W., Girod R., Poupardin R., Boyer F., Reynaud S., David J. P. (2017). In the hunt for genomic markers of metabolic resistance to pyrethroids in the mosquito Aedes aegypti : an integrated next-generation sequencing approach. Plos Neglected Tropical Diseases, 11 (4), p. e0005526 [20 p.]. ISSN 1935-2735.
Titre du document
In the hunt for genomic markers of metabolic resistance to pyrethroids in the mosquito Aedes aegypti : an integrated next-generation sequencing approach
Année de publication
2017
Auteurs
Faucon F., Gaude T., Dusfour I., Navratil V., Corbel Vincent, Juntarajumnong W., Girod R., Poupardin R., Boyer F., Reynaud S., David J. P.
Source
Plos Neglected Tropical Diseases, 2017,
11 (4), p. e0005526 [20 p.] ISSN 1935-2735
Background The capacity of Aedes mosquitoes to resist chemical insecticides threatens the control of major arbovirus diseases worldwide. Until alternative control tools are widely deployed, monitoring insecticide resistance levels and identifying resistance mechanisms in field mosquito populations is crucial for implementing appropriate management strategies. Metabolic resistance to pyrethroids is common in Aedes aegypti but the monitoring of the dynamics of resistant alleles is impeded by the lack of robust genomic markers. Methodology/Principal findings In an attempt to identify the genomic bases of metabolic resistance to deltamethrin, multiple resistant and susceptible populations originating from various continents were compared using both RNA-seq and a targeted DNA-seq approach focused on the upstream regions of detoxification genes. Multiple detoxification enzymes were over transcribed in resistant populations, frequently associated with an increase in their gene copy number. Targeted sequencing identified potential promoter variations associated with their over transcription. Non-synonymous variations affecting detoxification enzymes were also identified in resistant populations. Conclusion /Significance This study not only confirmed the role of gene copy number variations as a frequent cause of the over expression of detoxification enzymes associated with insecticide resistance in Aedes aegypti but also identified novel genomic resistance markers potentially associated with their cis-regulation and modifications of their protein structure conformation. As for gene transcription data, polymorphism patterns were frequently conserved within regions but differed among continents confirming the selection of different resistance factors worldwide. Overall, this study paves the way of the identification of a comprehensive set of genomic markers for monitoring the spatio-temporal dynamics of the variety of insecticide resistance mechanisms in Aedes aegypti.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020]
;
Entomologie médicale / Parasitologie / Virologie [052]
Description Géographique
BENIN ; POLYNESIE FRANCAISE ; THAILANDE ; ETATS UNIS ; GUYANE FRANCAISE
Localisation
Fonds IRD [F B010070100]
Identifiant IRD
fdi:010070100
