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Faucon F., Gaude T., Dusfour I., Navratil V., Corbel Vincent, Juntarajumnong W., Girod R., Poupardin R., Boyer F., Reynaud S., David J. P. (2017). In the hunt for genomic markers of metabolic resistance to pyrethroids in the mosquito Aedes aegypti : an integrated next-generation sequencing approach. Plos Neglected Tropical Diseases, 11 (4), e0005526 [20 p.]. ISSN 1935-2735

Fichier PDF disponible http://horizon.documentation.ird.fr/exl-doc/pleins_textes/divers17-06/010070100.pdf

Lien direct chez l'éditeur doi:10.1371/journal.pntd.0005526

Titre
In the hunt for genomic markers of metabolic resistance to pyrethroids in the mosquito Aedes aegypti : an integrated next-generation sequencing approach
Année de publication2017
Type de documentArticle référencé dans le Web of Science WOS:000402256700053
AuteursFaucon F., Gaude T., Dusfour I., Navratil V., Corbel Vincent, Juntarajumnong W., Girod R., Poupardin R., Boyer F., Reynaud S., David J. P.
SourcePlos Neglected Tropical Diseases, 2017, 11 (4), p. e0005526 [20 p.]. p. e0005526 [20 p.] ISSN 1935-2735
RésuméBackground The capacity of Aedes mosquitoes to resist chemical insecticides threatens the control of major arbovirus diseases worldwide. Until alternative control tools are widely deployed, monitoring insecticide resistance levels and identifying resistance mechanisms in field mosquito populations is crucial for implementing appropriate management strategies. Metabolic resistance to pyrethroids is common in Aedes aegypti but the monitoring of the dynamics of resistant alleles is impeded by the lack of robust genomic markers. Methodology/Principal findings In an attempt to identify the genomic bases of metabolic resistance to deltamethrin, multiple resistant and susceptible populations originating from various continents were compared using both RNA-seq and a targeted DNA-seq approach focused on the upstream regions of detoxification genes. Multiple detoxification enzymes were over transcribed in resistant populations, frequently associated with an increase in their gene copy number. Targeted sequencing identified potential promoter variations associated with their over transcription. Non-synonymous variations affecting detoxification enzymes were also identified in resistant populations. Conclusion /Significance This study not only confirmed the role of gene copy number variations as a frequent cause of the over expression of detoxification enzymes associated with insecticide resistance in Aedes aegypti but also identified novel genomic resistance markers potentially associated with their cis-regulation and modifications of their protein structure conformation. As for gene transcription data, polymorphism patterns were frequently conserved within regions but differed among continents confirming the selection of different resistance factors worldwide. Overall, this study paves the way of the identification of a comprehensive set of genomic markers for monitoring the spatio-temporal dynamics of the variety of insecticide resistance mechanisms in Aedes aegypti.
Plan de classementEntomologie médicale / Parasitologie / Virologie [052] ; Sciences fondamentales / Techniques d'analyse et de recherche [020]
Descr. géo.BENIN ; POLYNESIE FRANCAISE ; THAILANDE ; ETATS UNIS ; GUYANE FRANCAISE
LocalisationFonds IRD [F B010070100]
Identifiant IRDfdi:010070100
Lien permanenthttp://www.documentation.ird.fr/hor/fdi:010070100

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