@article{fdi:010070100,
  title = {{I}n the hunt for genomic markers of metabolic resistance to pyrethroids in the mosquito {A}edes aegypti : an integrated next-generation sequencing approach},
  author = {{F}aucon, {F}. and {G}aude, {T}. and {D}usfour, {I}. and {N}avratil, {V}. and {C}orbel, {V}incent and {J}untarajumnong, {W}. and {G}irod, {R}. and {P}oupardin, {R}. and {B}oyer, {F}. and {R}eynaud, {S}. and {D}avid, {J}. {P}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground {T}he capacity of {A}edes mosquitoes to resist chemical insecticides threatens the control of major arbovirus diseases worldwide. {U}ntil alternative control tools are widely deployed, monitoring insecticide resistance levels and identifying resistance mechanisms in field mosquito populations is crucial for implementing appropriate management strategies. {M}etabolic resistance to pyrethroids is common in {A}edes aegypti but the monitoring of the dynamics of resistant alleles is impeded by the lack of robust genomic markers. {M}ethodology/{P}rincipal findings {I}n an attempt to identify the genomic bases of metabolic resistance to deltamethrin, multiple resistant and susceptible populations originating from various continents were compared using both {RNA}-seq and a targeted {DNA}-seq approach focused on the upstream regions of detoxification genes. {M}ultiple detoxification enzymes were over transcribed in resistant populations, frequently associated with an increase in their gene copy number. {T}argeted sequencing identified potential promoter variations associated with their over transcription. {N}on-synonymous variations affecting detoxification enzymes were also identified in resistant populations. {C}onclusion /{S}ignificance {T}his study not only confirmed the role of gene copy number variations as a frequent cause of the over expression of detoxification enzymes associated with insecticide resistance in {A}edes aegypti but also identified novel genomic resistance markers potentially associated with their cis-regulation and modifications of their protein structure conformation. {A}s for gene transcription data, polymorphism patterns were frequently conserved within regions but differed among continents confirming the selection of different resistance factors worldwide. {O}verall, this study paves the way of the identification of a comprehensive set of genomic markers for monitoring the spatio-temporal dynamics of the variety of insecticide resistance mechanisms in {A}edes aegypti.},
  keywords = {{BENIN} ; {POLYNESIE} {FRANCAISE} ; {THAILANDE} ; {ETATS} {UNIS} ; {GUYANE} {FRANCAISE}},
  booktitle = {},
  journal = {{P}los {N}eglected {T}ropical {D}iseases},
  volume = {11},
  numero = {4},
  pages = {e0005526 [20 p.]},
  ISSN = {1935-2735},
  year = {2017},
  DOI = {10.1371/journal.pntd.0005526},
  URL = {https://www.documentation.ird.fr/hor/fdi:010070100},
}