Publications des scientifiques de l'IRD

Ortiz J. P. A., Revale S., Siena L. A., Podio M., Delgado L., Stein J., Leblanc Olivier, Pessino S. C. (2017). A reference floral transcriptome of sexual and apomictic Paspalum notatum. BMC Genomics, 18, p. art. 318 [14 p.]. ISSN 1471-2164.

Titre du document
A reference floral transcriptome of sexual and apomictic Paspalum notatum
Année de publication
2017
Type de document
Article référencé dans le Web of Science WOS:000400624200003
Auteurs
Ortiz J. P. A., Revale S., Siena L. A., Podio M., Delgado L., Stein J., Leblanc Olivier, Pessino S. C.
Source
BMC Genomics, 2017, 18, p. art. 318 [14 p.] ISSN 1471-2164
Background: Paspalum notatum Flugge is a subtropical grass native to South America, which includes sexual diploid and apomictic polyploid biotypes. In the past decade, a number of apomixis-associated genes were discovered in this species through genetic mapping and differential expression surveys. However, the scarce information on Paspalum sequences available in public databanks limited annotations and functional predictions for these candidates. Results: We used a long-read 454/Roche FLX+ sequencing strategy to produce robust reference transcriptome datasets from florets of sexual and apomictic Paspalum notatum genotypes and delivered a list of transcripts showing differential representation in both reproductive types. Raw data originated from floral samples collected from premeiosis to anthesis was assembled in three libraries: i) sexual (SEX), ii) apomictic (APO) and iii) global (SEX + APO). A group of physically-supported Paspalum mRNA and EST sequences matched with high level of confidence to both sexual and apomictic libraries. A preliminary trial allowed discovery of the whole set of putative alleles/paralogs corresponding to 23 previously identified apomixis-associated candidate genes. Moreover, a list of 3,732 transcripts and several co-expression and protein -protein interaction networks associated with apomixis were identified. Conclusions: The use of the 454/Roche FLX+ transcriptome database will allow the detailed characterization of floral alleles/paralogs of apomixis candidate genes identified in prior and future work. Moreover, it was used to reveal additional candidate genes differentially represented in apomictic and sexual flowers. Gene ontology (GO) analyses of this set of transcripts indicated that the main molecular pathways altered in the apomictic genotype correspond to specific biological processes, like biotic and abiotic stress responses, growth, development, cell death and senescence. This data collection will be of interest to the plant reproduction research community and, particularly, to Paspalum breeding projects.
Plan de classement
Sciences du monde végétal [076]
Localisation
Fonds IRD [F B010070019]
Identifiant IRD
fdi:010070019
Contact