@article{fdi:010070019,
  title = {{A} reference floral transcriptome of sexual and apomictic {P}aspalum notatum},
  author = {{O}rtiz, {J}. {P}. {A}. and {R}evale, {S}. and {S}iena, {L}. {A}. and {P}odio, {M}. and {D}elgado, {L}. and {S}tein, {J}. and {L}eblanc, {O}livier and {P}essino, {S}. {C}.},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground: {P}aspalum notatum {F}lugge is a subtropical grass native to {S}outh {A}merica, which includes sexual diploid and apomictic polyploid biotypes. {I}n the past decade, a number of apomixis-associated genes were discovered in this species through genetic mapping and differential expression surveys. {H}owever, the scarce information on {P}aspalum sequences available in public databanks limited annotations and functional predictions for these candidates. {R}esults: {W}e used a long-read 454/{R}oche {FLX}+ sequencing strategy to produce robust reference transcriptome datasets from florets of sexual and apomictic {P}aspalum notatum genotypes and delivered a list of transcripts showing differential representation in both reproductive types. {R}aw data originated from floral samples collected from premeiosis to anthesis was assembled in three libraries: i) sexual ({SEX}), ii) apomictic ({APO}) and iii) global ({SEX} + {APO}). {A} group of physically-supported {P}aspalum m{RNA} and {EST} sequences matched with high level of confidence to both sexual and apomictic libraries. {A} preliminary trial allowed discovery of the whole set of putative alleles/paralogs corresponding to 23 previously identified apomixis-associated candidate genes. {M}oreover, a list of 3,732 transcripts and several co-expression and protein -protein interaction networks associated with apomixis were identified. {C}onclusions: {T}he use of the 454/{R}oche {FLX}+ transcriptome database will allow the detailed characterization of floral alleles/paralogs of apomixis candidate genes identified in prior and future work. {M}oreover, it was used to reveal additional candidate genes differentially represented in apomictic and sexual flowers. {G}ene ontology ({GO}) analyses of this set of transcripts indicated that the main molecular pathways altered in the apomictic genotype correspond to specific biological processes, like biotic and abiotic stress responses, growth, development, cell death and senescence. {T}his data collection will be of interest to the plant reproduction research community and, particularly, to {P}aspalum breeding projects.},
  keywords = {{A}pomixis ; {N}ext generation sequencing ; {P}lant reproduction ; {S}exual reproduction ; {T}ranscriptomics},
  booktitle = {},
  journal = {{BMC} {G}enomics},
  volume = {18},
  numero = {},
  pages = {art. 318 [14 p.]},
  ISSN = {1471-2164},
  year = {2017},
  DOI = {10.1186/s12864-017-3700-z},
  URL = {https://www.documentation.ird.fr/hor/fdi:010070019},
}