Publications des scientifiques de l'IRD

Garba M., Loiseau A., Benoit L., Gauthier Nathalie. (2016). A new suite of twenty-two polymorphic microsatellite loci in the parasitic wasp, Habrobracon hebetor (Hymenoptera : Braconidae) : promising molecular tools for studying the population genetics of several beneficial braconid species. European Journal of Entomology, 113, p. 265-269. ISSN 1802-8829.

Titre du document
A new suite of twenty-two polymorphic microsatellite loci in the parasitic wasp, Habrobracon hebetor (Hymenoptera : Braconidae) : promising molecular tools for studying the population genetics of several beneficial braconid species
Année de publication
2016
Type de document
Article référencé dans le Web of Science WOS:000380706700030
Auteurs
Garba M., Loiseau A., Benoit L., Gauthier Nathalie
Source
European Journal of Entomology, 2016, 113, p. 265-269 ISSN 1802-8829
Combining a biotin-enrichment protocol and 454GS-FLX titanium pyrosequencing technology, we characterised 22 polymorphic microsatellite loci from the parasitic wasp, Habrobracon hebetor (Say) (Hymenoptera: Braconidae), a cosmopolitan species commonly used in biological control against a wide range of both major lepidopterous pests of stored products and field crops in different parts of the world. Three multiplex PCR sets were optimised and characterised across 46 H. hebetor specimens from two samples collected from millet fields in Niger. Two to 11 alleles were found per locus and observed heterozygosity ranged from 0.289 to 0.826. Polymorphism was detected in both samples with a similar level of observed heterozygosity (0.482 vs. 0.502) and number of alleles (4.1 vs. 3.6). Deviation from Hardy-Weinberg equilibrium was detected at the same five loci in both samples and five or seven more loci in each sample but was not associated with heterozygote deficiencies. Even though evidence for linkage disequilibrium was found between a few alleles, these new loci segregated independently. The variability of the 22 loci will enable estimates of genetic diversity and structure patterns, as well as gene flow between H. hebetor populations at different spatial scales. Cross-species amplifications were successful among the six Bracon spp. tested and nine loci will be particularly appropriate for population genetic studies in B. brevicornis.
Plan de classement
Sciences du monde végétal [076] ; Sciences du monde animal [080]
Description Géographique
NIGER
Localisation
Fonds IRD [F B010067725] ; Niamey (LASDEL)
Identifiant IRD
fdi:010067725
Contact