@article{fdi:010067725,
  title = {{A} new suite of twenty-two polymorphic microsatellite loci in the parasitic wasp, {H}abrobracon hebetor ({H}ymenoptera : {B}raconidae) : promising molecular tools for studying the population genetics of several beneficial braconid species},
  author = {{G}arba, {M}. and {L}oiseau, {A}. and {B}enoit, {L}. and {G}authier, {N}athalie},
  editor = {},
  language = {{ENG}},
  abstract = {{C}ombining a biotin-enrichment protocol and 454{GS}-{FLX} titanium pyrosequencing technology, we characterised 22 polymorphic microsatellite loci from the parasitic wasp, {H}abrobracon hebetor ({S}ay) ({H}ymenoptera: {B}raconidae), a cosmopolitan species commonly used in biological control against a wide range of both major lepidopterous pests of stored products and field crops in different parts of the world. {T}hree multiplex {PCR} sets were optimised and characterised across 46 {H}. hebetor specimens from two samples collected from millet fields in {N}iger. {T}wo to 11 alleles were found per locus and observed heterozygosity ranged from 0.289 to 0.826. {P}olymorphism was detected in both samples with a similar level of observed heterozygosity (0.482 vs. 0.502) and number of alleles (4.1 vs. 3.6). {D}eviation from {H}ardy-{W}einberg equilibrium was detected at the same five loci in both samples and five or seven more loci in each sample but was not associated with heterozygote deficiencies. {E}ven though evidence for linkage disequilibrium was found between a few alleles, these new loci segregated independently. {T}he variability of the 22 loci will enable estimates of genetic diversity and structure patterns, as well as gene flow between {H}. hebetor populations at different spatial scales. {C}ross-species amplifications were successful among the six {B}racon spp. tested and nine loci will be particularly appropriate for population genetic studies in {B}. brevicornis.},
  keywords = {{H}ymenoptera ; {B}raconidae ; {H}abrobracon hebetor ; {B}racon spp. ; biological control ; parasitic wasp ; microsatellite ; population genetics ; gene flow ; {NIGER}},
  booktitle = {},
  journal = {{E}uropean {J}ournal of {E}ntomology},
  volume = {113},
  numero = {},
  pages = {265--269},
  ISSN = {1802-8829},
  year = {2016},
  DOI = {10.14411/eje.2016.031},
  URL = {https://www.documentation.ird.fr/hor/fdi:010067725},
}