Vongsouvath M., Phommasone K., Sengvilaipaseuth O., Kosoltanapiwat N., Chantratita N., Blacksell S. D., Lee S. J., de Lamballerie Xavier, Mayxay M., Keomany S., Newton P. N., Dubot Pérès Audrey. (2016). Using rapid diagnostic tests as a source of viral RNA for dengue serotyping by RT-PCR : a novel epidemiological tool. Plos Neglected Tropical Diseases, 10 (5), p. e0004704 [15 p.]. ISSN 1935-2735.
Titre du document
Using rapid diagnostic tests as a source of viral RNA for dengue serotyping by RT-PCR : a novel epidemiological tool
Année de publication
2016
Auteurs
Vongsouvath M., Phommasone K., Sengvilaipaseuth O., Kosoltanapiwat N., Chantratita N., Blacksell S. D., Lee S. J., de Lamballerie Xavier, Mayxay M., Keomany S., Newton P. N., Dubot Pérès Audrey
Source
Plos Neglected Tropical Diseases, 2016,
10 (5), p. e0004704 [15 p.] ISSN 1935-2735
Background Dengue virus infection causes major public health problems in tropical and subtropical areas. In many endemic areas, including the Lao PDR, inadequate access to laboratory facilities is a major obstacle to surveillance and study of dengue epidemiology. Filter paper is widely used for blood collection for subsequent laboratory testing for antibody and nucleic acid detection. For the first time, we demonstrate that dengue viral RNA can be extracted from dengue rapid diagnostic tests (RDT) and then submitted to real-time RT-PCR for serotyping. Methodology/Principal Findings We evaluated the Standard Diagnostics (SD) Bioline Dengue Duo RDT, a commonly used test in dengue endemic areas. First, using the QIAamp RNA kit, dengue RNA was purified from the sample pad of the NS1 RDT loaded with virus isolates of the four serotypes, then quantified by RT-PCR. We observed greater recovery of virus, with a mean of 27 times more RNA recovered from RDT, than from filter paper. Second, we evaluated dengue NS1 RDTs from patients at Mahosot Hospital, Vientiane, (99 patients) and from rural Salavan Provincial Hospital (362 patients). There was good agreement between dengue RT-PCR from NS1 RDT with RT-PCR performed on RNA extracted from patient sera, either using RDT loaded with blood (82.8% and 91.4%, in Vientiane and Salavan, respectively) or serum (91.9% and 93.9%). There was 100% concordance between RDT and serum RTPCR of infecting dengue serotype. Conclusions/Significance Therefore, the collection of NS1 positive RDTs, which do not require cold storage, may be a novel approach for dengue serotyping by RT-PCR and offers promising prospects for the collection of epidemiological data from previously inaccessible tropical areas to aid surveillance and public health interventions.
Plan de classement
Entomologie médicale / Parasitologie / Virologie [052]
Description Géographique
LAOS
Localisation
Fonds IRD [F B010067590]
Identifiant IRD
fdi:010067590
