@article{fdi:010067590,
  title = {{U}sing rapid diagnostic tests as a source of viral {RNA} for dengue serotyping by {RT}-{PCR} : a novel epidemiological tool},
  author = {{V}ongsouvath, {M}. and {P}hommasone, {K}. and {S}engvilaipaseuth, {O}. and {K}osoltanapiwat, {N}. and {C}hantratita, {N}. and {B}lacksell, {S}. {D}. and {L}ee, {S}. {J}. and de {L}amballerie, {X}avier and {M}ayxay, {M}. and {K}eomany, {S}. and {N}ewton, {P}. {N}. and {D}ubot {P}{\'e}r{\`e}s, {A}udrey},
  editor = {},
  language = {{ENG}},
  abstract = {{B}ackground {D}engue virus infection causes major public health problems in tropical and subtropical areas. {I}n many endemic areas, including the {L}ao {PDR}, inadequate access to laboratory facilities is a major obstacle to surveillance and study of dengue epidemiology. {F}ilter paper is widely used for blood collection for subsequent laboratory testing for antibody and nucleic acid detection. {F}or the first time, we demonstrate that dengue viral {RNA} can be extracted from dengue rapid diagnostic tests ({RDT}) and then submitted to real-time {RT}-{PCR} for serotyping. {M}ethodology/{P}rincipal {F}indings {W}e evaluated the {S}tandard {D}iagnostics ({SD}) {B}ioline {D}engue {D}uo {RDT}, a commonly used test in dengue endemic areas. {F}irst, using the {QIA}amp {RNA} kit, dengue {RNA} was purified from the sample pad of the {NS}1 {RDT} loaded with virus isolates of the four serotypes, then quantified by {RT}-{PCR}. {W}e observed greater recovery of virus, with a mean of 27 times more {RNA} recovered from {RDT}, than from filter paper. {S}econd, we evaluated dengue {NS}1 {RDT}s from patients at {M}ahosot {H}ospital, {V}ientiane, (99 patients) and from rural {S}alavan {P}rovincial {H}ospital (362 patients). {T}here was good agreement between dengue {RT}-{PCR} from {NS}1 {RDT} with {RT}-{PCR} performed on {RNA} extracted from patient sera, either using {RDT} loaded with blood (82.8% and 91.4%, in {V}ientiane and {S}alavan, respectively) or serum (91.9% and 93.9%). {T}here was 100% concordance between {RDT} and serum {RTPCR} of infecting dengue serotype. {C}onclusions/{S}ignificance {T}herefore, the collection of {NS}1 positive {RDT}s, which do not require cold storage, may be a novel approach for dengue serotyping by {RT}-{PCR} and offers promising prospects for the collection of epidemiological data from previously inaccessible tropical areas to aid surveillance and public health interventions.},
  keywords = {{LAOS}},
  booktitle = {},
  journal = {{P}los {N}eglected {T}ropical {D}iseases},
  volume = {10},
  numero = {5},
  pages = {e0004704 [15 p.]},
  ISSN = {1935-2735},
  year = {2016},
  DOI = {10.1371/journal.pntd.0004704},
  URL = {https://www.documentation.ird.fr/hor/fdi:010067590},
}