Publications des scientifiques de l'IRD

Cassagnes L. E., Perio Pierre, Ferry G., Moulharat N., Antoine M., Gayon R., Boutin J. A., Nepveu F., Reybier K. (2015). In cellulo monitoring of quinone reductase activity and reactive oxygen species production during the redox cycling of 1,2 and 1,4 quinones. Free Radical Biology and Medicine, 89, p. 126-134. ISSN 0891-5849.

Titre du document
In cellulo monitoring of quinone reductase activity and reactive oxygen species production during the redox cycling of 1,2 and 1,4 quinones
Année de publication
2015
Type de document
Article référencé dans le Web of Science WOS:000366355800012
Auteurs
Cassagnes L. E., Perio Pierre, Ferry G., Moulharat N., Antoine M., Gayon R., Boutin J. A., Nepveu F., Reybier K.
Source
Free Radical Biology and Medicine, 2015, 89, p. 126-134 ISSN 0891-5849
Quinones are highly reactive molecules that readily undergo either one- or two-electron reduction. One-electron reduction of quinones or their derivatives by enzymes such as cytochrome P450 reductase or other flavoproteins generates unstable semiquinones, which undergo redox cycling in the presence of molecular oxygen leading to the formation of highly reactive oxygen species. Quinone reductases 1 and 2 (QR1 and QR2) catalyze the two-electron reduction of quinones to form hydroquinones, which can be removed from the cell by conjugation of the hydroxyl with glucuronide or sulfate thus avoiding its autoxidation and the formation of free radicals and highly reactive oxygen species. This characteristic confers a detoxifying enzyme role to QR1 and QR2, even if this character is strongly linked to the excretion capacity of the cell. Using [PR spectroscopy and confocal microscopy we demonstrated that the amount of reactive oxygen species (ROS) produced by Chinese hamster ovary (CHO) cells overexpressing QR1 or QR2 compared to naive CHO cells was determined by the quinone structural type indeed, whereas the amount of ROS produced in the cell was strongly decreased with para-quirmues such as menadione in the presence of quinone reductase 1 or 2, a strong increase in ROS was recorded with ortho -quinones such as adrenochrome, aminochrome, dopachrome, or 3,5-di-tert-butyl-o-benzoquinone in cells overexpressing QR, especially QR2. These differences could originate from the excretion process, which is different for para- and ortho-quinones. These results are of particular interest in the case of dopamine considering the association of QR2 with various neurological disorders such as Parkinson disease.
Plan de classement
Sciences fondamentales / Techniques d'analyse et de recherche [020] ; Santé : généralités [050]
Localisation
Fonds IRD [F B010066009]
Identifiant IRD
fdi:010066009
Contact