Markovic Z., Preiner D., Stupic D., Andabaka Z., Simon S., Voncina D., Maletic E., Kontic J. K., Chatelet P., Engelmann Florent. (2015). Cryopreservation and cryotherapy of grapevine (Vitis vinifera L.). Vitis, 54 (Special Issue), p. 247-251. ISSN 0042-7500.
Titre du document
Cryopreservation and cryotherapy of grapevine (Vitis vinifera L.)
Markovic Z., Preiner D., Stupic D., Andabaka Z., Simon S., Voncina D., Maletic E., Kontic J. K., Chatelet P., Engelmann Florent
Source
Vitis, 2015,
54 (Special Issue), p. 247-251 ISSN 0042-7500
This study aimed at testing the efficiency of a droplet-vitrification cryopreservation protocol in eliminating selected grapevine viruses. The cryopreservation protocol led to approximately 50 % recovery with cultivar 'Portan' and five international cultivars tested, but very low recovery was noted with Croatian cultivars. GFLV and GLRaV-3, two (economically important grapevine) viruses were eliminated in a high percentage (up to 100 %) of plants regenerated from cryopreserved shoot tips. Virus sanitation was observed as well in samples before liquid nitrogen exposure. Genetic stability of plants regenerated after cryopreservation was studied using AFLP markers. Polymorphic fragments were observed in non-cryopreserved and cryopreserved samples treated with PVS2 solution, the number of which increased with increasing durations of exposure to PVS2 solution.
Plan de classement
Sciences du monde végétal [076]
;
Biotechnologies [084]
