@article{fdi:010065505,
  title = {{C}ryopreservation and cryotherapy of grapevine ({V}itis vinifera {L}.)},
  author = {{M}arkovic, {Z}. and {P}reiner, {D}. and {S}tupic, {D}. and {A}ndabaka, {Z}. and {S}imon, {S}. and {V}oncina, {D}. and {M}aletic, {E}. and {K}ontic, {J}. {K}. and {C}hatelet, {P}. and {E}ngelmann, {F}lorent},
  editor = {},
  language = {{ENG}},
  abstract = {{T}his study aimed at testing the efficiency of a droplet-vitrification cryopreservation protocol in eliminating selected grapevine viruses. {T}he cryopreservation protocol led to approximately 50 % recovery with cultivar '{P}ortan' and five international cultivars tested, but very low recovery was noted with {C}roatian cultivars. {GFLV} and {GLR}a{V}-3, two (economically important grapevine) viruses were eliminated in a high percentage (up to 100 %) of plants regenerated from cryopreserved shoot tips. {V}irus sanitation was observed as well in samples before liquid nitrogen exposure. {G}enetic stability of plants regenerated after cryopreservation was studied using {AFLP} markers. {P}olymorphic fragments were observed in non-cryopreserved and cryopreserved samples treated with {PVS}2 solution, the number of which increased with increasing durations of exposure to {PVS}2 solution.},
  keywords = {grapevine ; cryopreservation ; cryotherapy ; genetic stability},
  booktitle = {},
  journal = {{V}itis},
  volume = {54},
  numero = {{S}pecial {I}ssue},
  pages = {247--251},
  ISSN = {0042-7500},
  year = {2015},
  URL = {https://www.documentation.ird.fr/hor/fdi:010065505},
}