Soumana I. H., Klopp C., Ravel Sophie, Nabihoudine I., Tchicaya B., Parrinello H., Abate Luc, Rialle S., Geiger Anne. (2015). RNA-seq de novo assembly reveals differential gene expression in Glossina palpalis gambiensis infected with Trypanosoma brucei gambiense vs. non-infected and self-cured flies. Frontiers in Microbiology, 6, p. art. 1259 [19 p.]. ISSN 1664-302X.
Titre du document
RNA-seq de novo assembly reveals differential gene expression in Glossina palpalis gambiensis infected with Trypanosoma brucei gambiense vs. non-infected and self-cured flies
Année de publication
2015
Auteurs
Soumana I. H., Klopp C., Ravel Sophie, Nabihoudine I., Tchicaya B., Parrinello H., Abate Luc, Rialle S., Geiger Anne
Source
Frontiers in Microbiology, 2015,
6, p. art. 1259 [19 p.] ISSN 1664-302X
Trypanosoma brucei gambiense (Tbg), causing the sleeping sickness chronic form, completes its developmental cycle within the tsetse fly vector Glossina palpalis gambiensis (Gpg) before its transmission to humans. Within the framework of an anti vector disease control strategy, a global gene expression profiling of trypanosome infected (susceptible), non-infected, and self-cured (refractory) tsetse flies was performed, on their midguts, to determine differential genes expression resulting from in vivo trypanosomes, tsetse flies (and their microbiome) interactions. An RNAseg de novo assembly was achieved. The assembled transcripts were mapped to reference sequences for functional annotation. Twenty-four percent of the 16,936 contigs could not be annotated, possibly representing untranslated mRNA regions, or Gpg- or Tbg-specific ORFs. The remaining contigs were classified into 65 functional groups. Only a few transposable elements were present in the Gpg midgut transcriptome, which may represent active transpositions and play regulatory roles. One thousand three hundred and seventy three genes differentially expressed (DEGs) between stimulated and non-stimulated flies were identified at day-3 post-feeding; 52 and 1025 between infected and self cured flies at 10 and 20 days post feeding, respectively. The possible roles of several DEGs regarding fly susceptibility and refractoriness are discussed. The results provide new means to decipher fly infection mechanisms, crucial to develop anti-vector control strategies.
Plan de classement
Entomologie médicale / Parasitologie / Virologie [052]
Description Géographique
AFRIQUE SUBSAHARIENNE
Localisation
Fonds IRD [F B010065491]
Identifiant IRD
fdi:010065491
